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热应激对小鼠早期囊胚发育能力和线粒体结构的影响 被引量:1

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摘要 对小鼠早期囊胚分别进行39℃和41℃2h的体外热应激处理,观察热应激后小鼠早期囊胚后续发育能力和线粒体结构的变化。结果41℃2h热应激显著降低了小鼠早期囊胚发育到扩张囊胚和孵化囊胚的百分率;39℃热应激组与正常培养组相比差异不显著(P>0.05)。热应激组扩张囊胚平均总细胞数均显著少于37℃正常培养组,孵化囊胚平均总细胞数与37℃正常培养组差异不显著(P>0.05)。39℃热应激组小鼠早期囊胚线粒体发生轻微肿胀,经37℃再培养2h后线粒体肿胀减轻;41℃热应激组胚胎线粒体高度肿胀,经37℃再培养2h后线粒体肿胀未减轻。上述结果说明,热应激后小鼠早期囊胚线粒体的损伤程度和修复能力决定了小鼠早期囊胚的后续发育能力。
出处 《上海畜牧兽医通讯》 2015年第6期2-4,共3页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
基金 山东省现代农业产业技术体系牛产业创新团队建设项目(SDAIT-06-011-05)
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