辅酶Q10纳米微乳的制备及其体外抗紫外辐射研究

Preparation of coenzyme Q10 micro emulsion and its in vitro anti ultraviolet radiation study

目的考察一种新的辅酶Q10纳米微乳制备工艺,并对其体外抗紫外辐射活性进行评价。方法采用高压均质法制备微乳,考察该微乳的p H值、粒径、Zeta电位、含量。进行长期稳定性实验,考察纳米微乳稳定性。观察该微乳对紫外损伤后人皮肤成纤维细胞、表皮细胞中活性氧(ROS)与基质金属蛋白酶1(MMP-1)水平的影响。结果采用聚氧乙烯氢化蓖麻油(RH40)2.5%,单硬脂酸三聚甘油酯(TGMS)2.5%,辅酶Q10 5%,水90.0%(W/W)配方形成均匀稳定的微乳系统;理化性质为:p H值(6.12±0.04),Zeta电位-(39.5±6.06)m V、平均粒径(225.2±23.76)nm、辅酶Q10含量为(5.55±0.35)%,可在室温下稳定放置12个月;该辅酶Q10微乳可显著降低紫外辐射(UVA、UVB)对皮肤成纤维细胞、表皮细胞的损伤,降低胞内ROS与细胞上清液中MMP-1水平(P<0.05)。结论本研究制备的辅酶Q10微乳分布均匀,理化指标稳定;体外试验证明该微乳具有一定的抗光老化效果。

Objective To investigate the preparation technology of a coenzyme Q10 and evaluate its UVB-Ultraviolet in vitro. Methods Coenzyme Q10-loaded micro emulsion was prepared by homogenizer method. The Coenzyme Q10 content was determined by HPLC. The micro emulsion features of drug globule size distribution, zeta potential, p H value were tested. A long time stability study was carried to verify micro emulsion stability. The effects of this micro emulsion on human skin fibroblast cells, ROS and MMP-1 levels in epidermis cells were observed. Results The optimum micro emulsion formulation consisted of 2.5% Cremophor RH40, 2.5% TGMS, 5% coenzyme q10, and 90.0% water based on weight percent. The average particle size of drug-loaded microemulsions was（225.2±23.76） nm, zetapotentialis was-（39.5±6.06） m V, p H was（6.12±0.04）, coenzyme Q10 content was（5.55±0.35）%. No significant changes in physical properties were recorded on storage for 12 months at room temperature. Coenzyme Q10 micro emulsion significantly decreased expression of UVR-induced（UVA, UVB） damage of fibroblasts and epidermiscells, intracellular ROS level and MMP-1level of cell supernatant（P〈0.05）. Conclusion The prepared coenzyme Q10 micro emulsion is equally distributed, has stable physicochemical property; In vitro tests shows that it has a certain effect of light aging resistance.