抗坏血酸对创伤性脑损伤的保护作用

The protective effect mechanisms about ascorbic acid on traumatic brain injury in rats

目的探讨抗坏血酸对颅脑创伤后引起的继发性脑损伤的保护作用。方法将实验大鼠随机分为假手术+生理盐水组、假手术+抗坏血酸组、打击+生理盐水组、打击+抗坏血酸组,每组12只。建立中度颅脑损伤模型,颅脑创伤后24h进行神经功能损伤评分,测定脑含水量及血脑屏障的通透性;采用Western blot法测定颅脑创伤后24 h的损伤侧脑组织中肿瘤坏死因子α(TNF-α)和水通道蛋白4(AQP-4)的表达情况。结果与打击+生理盐水组比较,打击+抗坏血酸组的神经功能损伤程度减轻(P<0.05),脑水肿程度降低(P<0.05),血脑屏障的破坏程度明显降低(P<0.05),TNF-α和AQP-4的含量降低(P<0.05)。结论抗坏血酸可以减轻打击后脑水肿和血脑屏障的破坏,起到脑保护的作用。

Objective To investigate the protective effect of ascorbic acid on secondary brain injury caused by traumatic brain injury and its possible mechanism. Methods The rats were randomly divided into sham ＋ saline group, sham ＋ ascorbic acid group, hit ＋ saline group and hit ＋ ascorbic acid groups. Moderate traumatic brain injury model was established, 24 hours after traumatic brain injury, the neurological injury score, brain water content and permea- bility of blood brain barrier were evaluated. 24 hours after traumatic brain injury, the expression of tumor necrosis factor-c~ （TNF-c0 and aquaporin 4 （AQP4） in damaged brain tissue were evaluated by Western blot method. Resuits Compared with the hit ＋ saline group, the hit ＋ ascorbic acid group＇s degree of neurologic injury mitigated and brain edema reduced, the extent of damage to blood brain barrier reduced significantly, TNF-α and AQP4 reduced. Conclusion Ascorbic acid can reduce brain edema and fight against the destruction of the blood brain barrier.