摘要
目的探讨FSCN1在胃癌增殖中的作用,并研究FSCN1与钌配合物在胃癌中的相互作用。方法通过荧光定量PCR及Western blot法检测胃癌组织中FSCN1的表达水平;MTT、Ed U法检测干扰FSCN1后细胞增殖能力变化;用钌配合物处理SGC-7901后检测FSCN1 mRNA、蛋白表达水平及细胞的增殖能力;siRNA抑制FSCN1的表达,过表达FSCN1后检测钌配合物对细胞的增殖能力影响。结果荧光定量PCR及Western blot法均显示胃癌组织中FSCN1表达水平显著高于癌旁组织;MTT与Ed U法结果显示,与si NC组比较,干扰FSCN1后细胞的增殖受到明显抑制(P<0.01);钌配合物处理SGC-7901后,定量PCR结果显示,与si NC组比较,FSCN1表达水平受到抑制(P<0.01),MTT法结果显示,与si NC组比较,细胞增殖受到抑制(P<0.01);MMT法结果显示过表达FSCN1后抑制钌配合物对细胞增殖能力的影响。结论 FSCN1具有促进胃癌细胞增殖的能力,钌配合物通过抑制FSCN1的表达起抑癌作用。
Objective To study the role of FSCN1 in the proliferation of gastric cancer and the interaction be- tween FSCN1 and Ru complex in gastric cancer. Methods The expression level of FSCN1 was measured by qRT- PCR and Western blot. The proliferation ability was tested using MTT and EdU assays. Then the mRNA and pro- tein levels of FSCN1 in SGC-7901 cells were measured after treating with Ru complex. FSCN1 were transfected into cells, and the proliferation ability was tested using MTT and EdU assays after treating with Ru complex. Results The expression level of FSCN1 in gastric cancer tissues was higher than that in adjacent tissues. Knockdown of FSCN1 in SGC-7901 cells inhibited cell proliferation. The proliferation ability and the expression level of FSCN1 in SGC-7901 cells were decreased after treating with Ru complex. And overexpression of FSCN1 reversed the anti- tumor effect of Ru complex. Conclusion FSCN1 as the oncogene can regulate gastric cancer proliferation, and Ru complex reverses the course by inhibiting FSCN1 expression.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第12期1744-1748,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省年度重点科研计划项目(编号:1301043022)
