水溶性纳米Fe_3O_4-阿魏酸抗凝血材料的合成及性能

Synthesis and Anticoagulant Properties of Water Soluble Nano-Oxides Fe_3O_4-Ferulic Acid Hybrid Material

为提高具抗凝血性能的阿魏酸分子在水中的溶解性进而提高其药效,利用DBI(3,4-二羟基苯甲醛)、PEG(聚乙二醇4000)和纳米Fe_3O_4,采用接枝的方法制备了水溶性纳米Fe_3O_4-DBI-PEG-阿魏酸抗凝血杂化材料,用IR、1H NMR、TG、SEM、TEM、VSM和粒度测试方法表征了产物。结果表明阿魏酸(FA)接枝在了经过DBI-PEG活化后的纳米Fe_3O_4氧化物表面。杂化材料具有良好的水溶性(溶解度大于10 mg·m L^(-1))和顺磁性。抗凝血试验表明相同条件下杂化材料的抗凝血时间和复钙时间比阿魏酸要长,杂化材料的活化部分凝血活酶时间(APTT)和凝血酶原时间(PT)比空白组要长,杂化材料的抗凝血时间随浓度的增大而延长。

A hybrid materials of Fe_3O_4-DBI-PEG-ferulic acid（DBI =3,4-bihydroxy benzaldehyde, PEG =polyethylene glycol） were prepared by the method of grafting. The materials were characterized by IR,~1H NMR,TG, SEM, TEM,VSM and particle size tests, which showed that the ferulic acid was grafted on the surface of Fe_3O_4nano-oxides which was modified by DBI-PEG. Meanwhile, the hybrid materials are dissoluble in water（solubility10 mg·m L^（-1））and with paramagnet. The anticoagulant properties of ferulic acid and the hybrid materials were tested by coagulation time（CT）, recalcification time（RT）, activated partial thromboplastin time（APTT）, and prothrombin time（PT）. The results indicated that the hybrid materials have better anticoagulant action than that of ferulic acid.