白细胞介素2、6和17水平与甲状腺相关性眼病患者病情活动度的相关性研究

Association of serum IL-2, IL-6, and IL-17 levels with the activity of thyroid-associated ophthalmopathy

目的 观察甲状腺相关性眼病（TAO）患者血清白细胞介素（IL）-2、IL-6及IL-17水平与病情活动程度的相关性.方法 选取TAO患者80例,单纯Graves病（GD）患者30例,健康对照者（NC）30名.按临床活动性评分（CAS）将TAO患者分为非活动期（CAS＜3,27例）和活动期（CAS≥3,53例）,其中活动期中重度患者45例给予激素静脉治疗12周.测定各组基线或治疗后IL-2、IL-6及IL-17水平.结果 IL-2水平在TAO组、GD组和NC组依次升高（P＜0.05）;IL-6水平TAO组与GD组相似（P＞0.05）,但均高于NC组（P＜0.01）;IL-17水平在TAO组、GD组和NC组依次降低（P＜0.05）;与非活动期相比,活动期TAO患者IL-17水平升高（P＜0.01）,IL-2和IL-6水平无显著变化（P＞0.05）.TAO患者IL-17水平与CAS、促甲状腺素受体抗体呈正相关（P＜0.01）.使用激素治疗后,TAO患者CAS降低（P＜0.05）,IL-6、IL-17水平下降（P＜0.05）,IL-2水平无显著变化（P＞0.05）.结论 TAO患者血清IL-6、IL-17水平升高,IL-2水平降低;其中IL-17与病情活动度关系密切.

Objective To investigate the involvement of interleukin（IL）-2, IL-6, and IL-17 in the disease activity of thyroid-associated ophthalmopathy （TAO）.Methods The baseline serum levels of interleukin （IL）-2, IL-6, and IL-17, measured by enzyme-1 inked immunosorbent assay, were compared among 80 TAO patients, 30 Graves＆#39; disease （GD） patients without ophthalmopathy, and 30 healthy subjects （NC） to determine the TAO-associated changes of cytokines.Based on clinical activity score （CAS）, TAO patients were divided into non-active or active groups.Then, a cohort of 45 patients with active moderate-to-severe TAO received iv methylprednisolone therapy for 12 weeks, and changes in serum cytokines were evaluated.Results Serum IL-2 levels were gradually increased in NC, GD, and TAO groups （P〈0.05）, while serum IL-17 levels were decreased （P〈0.05）.Serum levels of IL-6 were similar in GD and TAO groups（P〉0.05）, but higher compared with NC group （P〈0.05）.Serum IL-17 levels were higher in active TAO patients than that in inactive TAO patients （P〈0.01）.However, there were no significant differences in IL-2 and IL-6 levels between active and inactive groups （P〉0.05）.The serum levels of IL-17 were positively related with CAS and thyrotropin receptor antibody （P〈0.01）.After methylprednisolone therapy for 12 weeks, significant decreases of IL-6 and IL-17 levels compared to their baseline levels（P〈0.05） were observed while IL-2 showed no significant change.Conclusion The serum level of IL-2 was decreased in TAO patients, while the serum levels of IL-6 and IL-17 were increased.In addition, IL-17 might serve as a useful biomarker for evaluating TAO activity.