骨髓内皮祖细胞移植治疗糖尿病大鼠周围神经病变的疗效观察

Effect of bone marrow-derived endothelial progenitor cells on peripheral neuropathy of diabetic rats

目的 观察大鼠骨髓内皮祖细胞（bone marrow-derived endothelial progenitor cells, EPCs）移植是否对糖尿病大鼠坐骨神经病变有治疗作用.方法 用链脲佐菌素60 mg/kg腹腔注射制备糖尿病大鼠.将雄性SD大鼠分为正常组（NC组）、糖尿病周围神经病变（diabetic peripheral neuropathy,DPN）组（DPN组）、DPN＋生理盐水组（DPN＋S组）和DPN＋EPCs组.用肌电图与诱发电位仪测定坐骨神经运动神经传导速度（motor nerve conduction velocity,MNCV）,在光镜和电镜下观察坐骨神经病理学改变.结果 与NC组[（52.91 ±4.53） m/s]相比,DPN组、DPN＋S组大鼠的坐骨神经MNCV明显减慢[（36.51±6.30对25.37±5.48）m/s,P＜0.01].与DPN组相比,DPN＋EPCs组的MNCV明显提高[（36.51±6.30对46.20±11.70）rn/s,P＜0.01].光镜和电镜观察发现：DPN组、DPN＋EPCs组和DPN＋S组坐骨神经均有脱髓鞘及轴索萎缩等病理改变;与DPN＋S组相比,DPN＋EPCs组的病变程度较轻.结论 糖尿病大鼠成模12周时能成功构建DPN大鼠.EPCs移植能改善DPN大鼠的坐骨神经MNCV、脱髓鞘及轴索萎缩等病理学改变.

Objective To explore whether endothelial progenitor cells （EPCs） exerts therapeutic effects on rats with diabetic peripheral neuropathy （DPN）.Methods Diabetes was induced by streptozotocin.Male SD rats were divided into normal control group（NC group）, diabetic peripheral neuropathy group（DPN group）, DPN＋saline group（DPN＋S group）, and DPN＋EPCs group.Sciatic nerve motor nerve conduction velocity（MNCV） was measured by a electromyography and trigger potentiometer instrument.Pathological changes were observed under optical microscope and electron microscope.Results Compared with normal control group [（52.91 ± 4.53） m/s], both DPN group and DPN＋S group had slower sciatic nerve MNCV [（36.51 ± 6.30 and 25.37 ± 5.48） m/s, P〈0.01].Compared with DPN group, the MNCV of sciatic nerve in DPN ＋ EPCs group had improved significantly [（36.51 ± 6.30 vs 46.20 ± 11.70） m/s, P 〈 0.01].Sciatic nerve pathological changes, characterized by demyelination and axonal degeneration under light microscope and electron microscope were observed in DPN group, DPN＋EPCs group, and DPN＋S group.Compared with DPN＋S group, the sciatic nerve pathological changes of DPN＋EPCs group were alleviated.Conclusion After fed for 12 weeks, diabetic rats could progress to DPN rats.Transplantation of EPCs could improve MNCV, demyelination and axonal degeneration changes of sciatic nerver of the DPN rats.