蛹虫草基质多糖对酒精所致雄性小鼠亚急性肝损伤的影响

Effect of Polysaccharides from Cordyceps militaris stroma against Alcohol-Induced Sub-Acute Liver Injury in Male Mice

目的研究蛹虫草基质多糖(Cordyceps militaris stroma polysaccharides,CMSP)对酒精所致雄性小鼠亚急性肝损伤的影响。方法雄性ICR小鼠被随机分为空白对照组、模型组、阳性对照组[150mg/(kg·d)]、CMSP各剂量组[150、300、600mg/(kg·d)]。除空白对照组外,其余各剂量组分别在灌胃蒸馏水、CMSP及联苯双酯后6h灌胃30%体积分数的乙醇溶液,连续灌胃28d。处死所有小鼠,测定血浆中葡萄糖(glucose,GLU)含量、总胆红素(total bilirubin,TBIL)含量、谷丙转氨酶(alanine aminotransferase,ALT)活性、谷草转氨酶(aspartate aminotransferase,AST)活性、碱性磷酸酶(alkaline phosphatase,ALP)活性、甘油三酯(triglyceride,TG)含量、总胆固醇(total cholesterol,CHOL)含量以及乳酸脱氢酶(lactic acid dehydrogenase,LDH)活性,取肝脏计算其指数,并制备肝匀浆测定其中乙醇脱氢酶(alcohol dehydrogenase,ADH)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、丙二醛(malondialdehyde,MDA)和还原型谷胱甘肽(glutathione transferase,GSH)的含量,并观察肝组织病理学变化。结果 CMSP各剂量组肝脏系数、血浆中GLU、TBIL、TG、CHOL含量、ALT、AST、ALP、LDH活性及肝组织中ADH、GSH-Px活力、MDA、GSH含量与模型组各水平相比均具有显著性差异(P<0.05或P<0.01)。结论蛹虫草基质多糖对酒精诱导的小鼠亚急性肝损伤有明显的保护作用。

Objective To explore the effect of Cordyceps militaris stroma polysaccharides （CMSP） on alcohol - induced sub - a- cute liver injury in male mice. Methods Mice were randomly divided into blank control, alcohol -induced liver injury model, posi- tive control and CMSP treatment [ at doses of 150,300 and 600 mg/（ kg · d） ] groups. Animals were administered orally with 30% alcohol at 6 h after the doses of CMSP everyday for twenty - eight consecutive days except mice in normal group. All animals were sacrificed, and blood were collected for the measurement of glucose （GLU）content, total bilirubin （TBIL）content, alanine transami- nase （ALT） activity, aspartate transaminase （AST） actinity, alkaline phosphatase （ALP） acitivty, triglyceride （TG） content, total cho- lesterol（CHOL） content, and lactic acid dehydrogenase（LDH） activity in serum, the livers were collected to calculate the coeffi- cients, preparation of liver homogenate were measured the activities of alcohol dehydrogenase （ADH） activity, glutathione peroxi- dase （ GSH - Px） activity, malondialdehyde （MDA） and glutathione （GSH） content, and pathological observation of liver tissue. Re- suits The liver coefficients, serum GLU, TBIL,TG and CHOL contents, and ALT, AST, ALP and LDH activities, and ADH activi- ty, GSH - Px activity, MDA and GSH contents in the liver of mice from the CMSP groups were significantly different to those of the liver injury model group （P 〈 0.05 or P 〈 0. 01 ）. Conclusion CMSP has significantly hepatoprotective effect on alcohol -induced sub - acute liver injury in mice.