急性缺血性脑卒中患者血清miR-106b-5p的异常表达及意义

Abnormal expression of serum miR-106b-5p in acute ischemic stroke patients and its clinical significance

目的检测急性缺血性脑卒中（IS）患者血清miR-106b-5p表达水平,探讨其作为IS分子诊断指标的可行性。方法用miRNA表达谱芯片筛选4例急性IS及10例健康体检者（NC）血清中差异表达的miRNAs。用实时荧光定量PCR法检测53例急性IS患者及50例NC血清中miR-106b-5p的含量。通过分析受试者工作特征曲线（ROC）判断miR-106b-5p在急性IS诊断中的敏感性和特异性。结果 IS组患者血清miR-106b-5p含量（67.13±4.13）fmol/L高于NC组（40.48±2.62）fmol/L,差异有统计学意义（t=5.41,P〈0.01）。miR-106b-5p诊断急性IS的ROC曲线下面积为0.78（95%CI：0.69-0.87）。当cut off值为40 fmol/L时,miR-106b-5p诊断急性IS的敏感性为79.25%,特异性为64.71%。结论 miR-106b-5p在IS患者血清中表达水平升高,与临床症状结合可提高诊断的准确性。

Objective To detect the levels of serum miR-106b-5p in acute ischemic stroke（ IS） patients and evaluate its potential as a molecular biomarker for the diagnosis of IS. Methods Serum miRNA profiles in four acute IS patients and ten healthy volunteers were screened by miRNA microarray analysis. Then,the levels of serum miR-106b-5p in 53 acute IS patients and 50 healthy controls were detected with quantitative real-time polymerase chain reaction. Next,the receiver operating characteristic（ ROC） curves were drawn to analyze the sensitivity and specificity of miR-106b-5p in the diagnosis of acute IS. Results The expression levels of serum miR-106b-5p in IS patients were significantly higher than that in healthy controls（ [67. 13 ± 4. 13]fmol / L vs [40. 48 ± 2. 62]fmol / L,t = 5. 41,P〈0. 01）. The area under the ROC curve of miR-106b-5p for the diagnosis of acute IS was 0. 78（ 95% CI： 0. 69-0. 87）. When the cutoff value was 40 fmol / L,the sensitivity and specificity of miR-106b-5p for the diagnosis of acute IS were 79. 25% and 64. 71%,respectively. Conclusion The levels of serum miR-106b-5p in acute IS patients increased obviously,which may improve the accuracy of the diagnosis of IS when combined with clinical symptoms of IS patients.