吉林地区人群戊型肝炎病毒感染的流行病学调查

Prevalence of the hepatitis E virus in people in the Jilin area of China

目的调查吉林地区部分人群戊型肝炎病毒(HEV)感染情况。方法用ELISA检测人群血清中抗HEV抗体;对部分血清用RT-PCR检测HEV RNA,对PCR阳性产物进行测序,所得序列构建系统发育树。结果 ELASA方法检测7 514份人血清,1 621份抗HEV抗体阳性,阳性率21.57%。其中男、女性阳性率分别为30.87%和12.01%,男、女性感染者之比为2.64︰1。感染者抗体水平随年龄增加而升高。不同职业人群HEV率不同,以猪销售人员及养猪从业人员感染率最高,分别为97.18%和97.09%。从戊型肝炎患者中检测HEV RNA,序列分析结果为HEV基因Ⅳ型。结论吉林地区人群HEV感染率较高。从进化关系角度分析,吉林地区人源HEV与猪源HEV可能分化于同一分支。

Objective To investigate the prevalence of the hepatitis E virus（HEV）in people in Jilin. Methods AntiHEV antibodies were detected with ELISA and HEV RNA was detected with RT-PCR.PCR products testing positive for HEV RNA were sequenced,and the sequences were analyzed. Results Of 7 514 serum samples,1 621 tested positive for anti-HEV antibodies at a rate of 21.57%.Males tested positive at a rate of 30.87% and females tested positive at a rate of 12.01%.The ratio of males to females who tested positive was 2.64：1.The anti-HEV antibody titer increased with age.The prevalence of HEV differs among occupations.Sequencing of HEV RNA（348bp）indicated that the HEV was genotype IV. Conclusion The human HEV found in Jilin is highly similar to the swine HEV found in Jilin（the latter is also genotype IV）.In a phylogenetic analysis,they fell on the same branch.