汉防己甲素对大鼠脊髓损伤后炎症介质及巢蛋白表达的影响

Effect of tetrandrine on expressions of inflammatory mediators and nestin after spinal cord injury in rats

目的．探讨汉防己甲素（Tet）对大鼠脊髓损伤（SCI）后炎症介质及其对内源性神经干细胞（ENSCs）增殖的影响。方法选用162只Wistar大鼠，按随机数字表法分为损伤组、代t治疗组和假手术组，每组54只。损伤组、Tet治疗组采用Allen’s打击法制备SCI动物模型，Tet治疗组于伤后30min、24h、48h尾静脉注射Tet22．5mg／kg，损伤组、假手术组注射同等剂量的等渗盐水。于伤后6，12h、1，3，5d、1周取伤段脊髓组织用于肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、IL-10的检测；于伤后1，5d、1，2，5，4周取伤段脊髓组织用于HE染色及巢蛋白免疫组化检测。结果伤后6，12h、1，3，5d、1周损伤组和Tet治疗组TNF-α、IL-1β、IL-10含量高于假手术组（P〈0．05）；Tet治疗组IL一10含量高于损伤组，而TNF-α、IL-1β含量低于损伤组。损伤组及Tet治疗组巢蛋白阳性细胞数在伤后1，3d、1，2，3，4周时与假手术组比较，差异有统计学意义（P〈0．05）。Tet治疗组表达数量高于损伤组，两组伤后1，3d、1，2，3周时巢蛋白阳性细胞数量差异有统计学意义（P〈0．05）。结论SCI后Tet治疗可减轻局部炎症反应，增加神经干细胞的数量，有利于神经功能的恢复。

Objective To measure the effect of tetrandrineon （Tet） on inflammatory mediators and endogenous neural stem cell proliferation after spinal cord injury （SCI） in rats. Methods A total of 162 Wistar rats were separated into injury group, Tet group and sham operation group according to the random number table, with 54 rats per group. Allen＇ s method was used for induction of experimental SCI. Animals in Tet group were given Tet （22.5 mg/kg） through the tail vein at 30 min, 24 h and 48 h postinjury. The same volume of normal saline was given to other two groups. Spinal cord tissue samples were taken from the rats after injury to measure levels of tumor necrosis factor （TNF）- α, interleukin （IL）-1β and IL-10, and tissues were examined with HE staining and Nestin immunohistochemistry staining. Results Levels of TNF- α, IL-1 Band IL-10 in injury and Tet groups increased compared to these in sham operation group at6 h, 12 h, 1 d, 3 d, 5 d and 1 week postinjury （P〈0.05）. At the same time point, level of IL-IO was higher in Tet group than in injury group, but inversely for TNF- α and IL-1β （P 〈 0.05 ）. More Nestin- positive cells were present in injury and Tet groups than in sham operation group at 1 d, 3 d, 1 week, 2 week, 3 week and 4 week postinjury （P 〈 0.05）. Additionally, more Nestin - positive cells were found in Tet group than in injury group at 1 d, 3 d, 1 week, 2 week and 3 week postinjury （ P 〈 0.05 ）. Conclusion Tet is effective to relieve inflammatory reaction, increase neural stem cell number and promote neurological recovery after SCI.