血浆miRNA-372/373在正常献血者和HBV早期感染献血者中的表达差异

Differential expression of mi RNA-372/373 in plasma between the normal donors and the HBV donors at early stage

目的:研究miRNA-372/373与HBV早期感染的相关性,分析其在HBV早期感染诊断中的潜在应用价值。方法:收集来源于2013年3月至9月天津市内无偿献血者所献血液的血浆标本留样,共54份,分为正常组、酶免单阳组(E单阳组)和酶免核酸双阳组(EN双阳组)。提取此3组共54份血浆标本中的mi RNA-372和miRNA-373,用实时荧光定量PCR方法进行检测。分别比较mi RNA-372和mi RNA-373在3组血浆标本之间的表达差异,并对各组间的mi RNA表达趋势进行统计学分析;同时,对miRNA-372、miRNA-373的相对表达水平分别与核酸检测HBV核酸阳性血浆标本中的HBV DNA拷贝数进行相关性分析。结果:经实时荧光定量PCR检测,与正常献血者相比,miRNA-372、miRNA-373在E单阳组和EN双阳组中均表达上调,其中,mi RNA-372、mi RNA-373在EN双阳组中表达上调更为显著。进一步分析发现,在EN双阳组中,mi RNA-372、miRNA-373均与相应的HBV DNA拷贝数呈正相关性,且与mi RNA-372相比,mi RNA-373与相应的HBV DNA拷贝数的正相关性更为明显。结论:miRNA-372、miRNA-373与HBV早期感染密切相关,提示miRNA-372、miRNA-373在HBV感染的早期诊断中具有潜在价值。

Objective：To explore the relationship between the miRNA-372/373 levels in plasma and the early-stage infection of HBV, to uncover the potential of miRNA-372/373 in the diagnosis of the early-stage infection of HBV. Methods： Blood samples for miRNA detecting were collected from 54 donors who donated blood from 2013.3 to 2013.9 in Tianjin. All the samples were divided into 3 groups：eighteen samples were Normal;18 samples were ELISA testing （＋） and NAT （-） （HBV-S）;the last 18 samples were ELISA testing （＋） and NAT（＋） （HBV-D）. Quantization of miRNA-372 and miRNA-373 in all the 54 plasma samples were performed using quantitative RT-PCR. At last, compared thederegulated miRNA （miRNA-372/-373） in the plasma of the three groups respectively, and the expression patterns of miRNA-372 and miRNA-373 were analyzed using statistical methods;the correlation between the expression level of miRNA-372/-373 and the copy number of HBV DNA in NAT （＋） plasma was statistically analyzed. Results：Compared with the Normal, miRNA-372 and miRNA-373 in both HBV-S and HBV-D were up-regulated. Furthermore, both miRNA-372 and miRNA-373 were up-regulated more significantly in HBV-D than in HBV-S. Moreover, the levels of both miRNA-372 and miRNA-373 were positively correlated with the copy number of HBV DNA in HBV-D. Compared with miRNA-372, miRNA-373’s positive correlation with the copy number of HBV DNA was significantly higher. Conclusion： The correlation between miRNA-372/373 in plasma and the early-stage infection of HBV is high , indicating their potential values in the early-stage diagnosis of HBV infection.