UPLC法同时测定藿香正气滴丸中7个化学成分

Simultaneous determination of seven constituents in Huoxiang Zhengqi Dripping Pill by UPLC

目的建立UPLC法同时测定藿香正气滴丸中欧前胡素、异欧前胡素、橙皮苷、和厚朴酚、厚朴酚、苍术素、甘草酸7个成分的含量。方法采用Waters Acquity UPLC BEH C18色谱柱（100 mm×2.1 mm,1.7μm）,流动相：乙腈（A）-体积分数为0.2%的甲酸水溶液（B）,梯度洗脱,流速：0.4 m L·min^-1,柱温：30℃,检测波长：250 nm,检测欧前胡素、异欧前胡素和甘草酸,284 nm检测橙皮苷,290 nm检测和厚朴酚、厚朴酚,336 nm检测苍术素。结果欧前胡素、异欧前胡素、橙皮苷、和厚朴酚、厚朴酚、苍术素、甘草酸质量浓度分别在2.0-80.6、0.6-23.0、15.0-600.0、10.1-403.2、7.5-301.6、1.0-41.6、10.1-202.4 mg·L^-1内与峰面积呈良好的线性关系（r≥0.999 5）,平均回收率在98.6%-100.9%（RSD≤2.2%,n=9）。结论该方法可用于藿香正气滴丸的质量控制。

Objective To establish a UPLC method for the simultaneous determination of seven constituents,including imperatorin,isoimperatorin,hesperidin,honokiol,magnolol,atrctylodine and glycyrrhizic acid in Huoxiang Zhengqi Dripping Pill（ HZDP）. Methods The UPLC separation was achieved on an Waters Acquity UPLC BEH C18column（ 100 mm × 2. 1 mm,1. 7 μm） with acetonitrile（ A）-0. 2% formic acid solution（ B） as mobile phase at the flowrate of 0. 4 m L·min^- 1for gradient elution. The column temperature was30 ℃. The UV detection wavelengths were set at 250 nm for imperatorin,isoimperatorin and glycyrrhizic acid,284 nm for hesperidin,290 nm for honokiol and magnolol,336 nm for atrctylodine. Results The linear ranges of imperatorin,isoimperatorin,hesperidin,honokiol,magnolol,atrctylodine and glycyrrhizic acid were2. 0-80. 6,0. 6-23. 0,15. 0-600. 0,10. 1-403. 2,7. 5-301. 6,1. 0-41. 6 and 10. 1-202. 4 mg·L^- 1,respectively.The average recoveries（ n = 9） of imperatorin,isoimperatorin,hesperidin,honokiol,magnolol,atrctylodine and glycyrrhizic acid were between 98. 6% and 100. 9% with RSD less than 2. 2%. Conclusions The method is simple,accurate,and could be used for the quality control of HZDP.