七氟醚后处理对成熟兔心肌缺血再灌注后IL-8、IL-10浓度及心肌组织超微结构的影响

Effect of Sevoflurane Postconditioning on the Levels of IL-IL-8 and IL-IL-10 and on the Ultramicrostructure of Myocardial Tissue after Myocardial Ischemia Reperfusion in Mature Rabbits

目的研究七氟醚后处理对成熟兔心肌缺血再灌注后IL-8、IL-10浓度及心肌超微结构的影响。方法选32只新西兰成熟兔随机分为缺血再灌注组（I/R组）与七氟醚后处理组（S组）,每组16只。I/R组结扎冠状动脉前降支缺血30 min,再灌注240 min。S组结扎冠状动脉前降支缺血30 min,再灌注即刻吸入2.5%七氟醚30 min,洗脱10 min,再灌注持续至240 min。监测缺血前（T1）、再灌注后1h（T2）、2h（T3）、4h（T4）血浆IL-8、IL-10浓度,观察心肌超微结构变化。结果 T2~T4时两组IL-8、IL-10均高于T1时（P〈0.05）,两组组内T1时分别比较IL-8、IL-10,差异无统计学意义（P〉0.05）;S组T2~T4时IL-8表达明显低于IR组（P〈0.05）,而IL-10表达明显高于IR组。显微镜下S组超微结构损伤较I/R组轻。结论七氟醚后处理可抑制缺血再灌注后心肌致炎性介质IL-8的过渡表达,增强抗炎性介质IL-10的表达,调节致炎抗炎平衡,减轻缺血再灌注心肌超微结构的损伤。

Objective To explore the effect of sevoflurane postconditioning on the levels of IL-8 and IL-10 and on the ultramicrostructure of myocardial tissue after myocardial ischemia reperfusion（MIR） in mature rabbits. Methods 32 mature New Zealand rabbits were randomly divided into 2 groups： I/R group（ischemia reperfusion group） and S group（sevoflurane postconditioning group）, 16 in each; the anterior descending coronary artery occlusion for 30 minutes followed by reperfusion for 240 minutes was made to rabbits in I/R group while to rabbits in S group, the anterior descending coronary artery occlusion for 30 minutes followed by reperfusion for 30 minutes with immediate inhalation of 2.5% sevoflurane was first made and then an elution for 10 minutes followed by reperfusion for240 minutes; a detection was made to the level of IL-8 and IL-10 at the time point of before ischemia（T1）, 1 hour after reperfusion（T2）,2 hours after reperfusion（T3） and 4 hours after reperfusion（T4） while an observation was made to the changes of ultramicrostructure of myocardial tissue at each time point in both groups. Results The levels of IL-8 and IL-10 at T2 and T4in both groups were higher than those at T1（P〈0.05） while no difference of statistical significance was found in the levels of IL-8 and IL-10 at T1 from those at T2 and T4in each group; the levels of IL-8 at T2 and T4in S group were much lower than those in I/R group（（P〈0.05） while the level of IL-10 in S group was much higher than that in I/R group; microscopic examination showed a slighter myocardial ultramicrostructural injury in S group than in I/R group. Conclusions Sevoflurane postconditioning can inhibit the over-expression of IL-8, enhance the expression of IL-10, regulate the balance of inflammatory and anti-inflammatory cytokines expression and reduce the myocardial ultramicrostructural injury after ischemia-reperfusion.