双水相法分离螺旋藻藻蓝蛋白与多糖

The extraction of C-phycocyanin and polysaccharides from Spirulina using aqueous two-phase system

以螺旋藻为原料,采用PEG2000-硫酸镁双水相体系对螺旋藻粗提液中的藻蓝蛋白和多糖进行分离。分别探讨了PEG2000质量分数、硫酸镁质量分数、离子强度、体系pH及萃取次数对双水相体系萃取藻蓝蛋白与多糖的影响,设计正交试验优化萃取条件,并考察了螺旋藻的反萃取体系条件。结果表明:当硫酸镁质量分数为14%,PEG质量分数为6%,KCl质量分数为0.5%,体系为p H4.0时,为优化双水相萃取体系。螺旋藻水提液通过双水相萃取,藻蓝蛋白富集于上相,萃取率为93.91%;下相中糖类萃取率为59.58%。收集上相,采用12%PEG,6%硫酸镁的双水相体系对藻蓝蛋白进行反萃取,藻蓝蛋白反萃取率为99.06%。螺旋藻水提液经过PEG2000-硫酸镁双水相体系萃取与反萃取,藻蓝蛋白回收率达92.66%,藻蓝蛋白纯度由0.78提高到2.64。

PEG2000-MgSQ aqueous two-phase extraction （ATPE） was used to isolate C-phycocyanin and polysaccharides from crude extract of Spirulina. The influence of various process parameters on the isolation and purification of C-phyeoeyanin and polysaccharides was studied, such as mass fraction of PEG2000, magnesium sulfate, neutral salts and system pH, and the orthogonal experiment was designed to optimize conditions. The reverse extraction of phycoeyanin was also studied. The results showed that the optimal ATPE conditions were 14% MgSO4, 6% PEG2000, 0.5% KCI, system pH 4.0. After the optimal ATPE, the yield of CPC was 93.91% from the top phase, and saecharides yield were 59.58% from the bottom phase. The optimal reverse extraction condition of CPC was 12% PEG2000, 6% MgSO4. After extraction and reverse extraction, 92.66% of CPC was obtained. Meanwhile, the purity of CPC was increased to 2.64 from initial 0.78.