影响空弯菌抗原诱导自身免疫应答的因素

Influences of both genetic background and sex on the production of autoantibodies induced by vaccine of campylobacter jejuni

利用慢性粘膜免疫应答小鼠模型,研究在空弯菌抗原(CJ-S131菌苗)诱导下,不同品系、性别的小鼠的自身免疫应答。经肠道,持续用CJ-S131菌苗免疫16周后,用ELISA或CELISA检测小鼠体内的抗ds-DNA,ss-DNA,组蛋白,ENA和胸腺细胞抗体。在五个品系小鼠中,四个品系小鼠(BALB/C,ICR,KM和SMMC/B)均有一种或多种被测的自身抗体升高。其中SMMC/B和KM小鼠的EI值较高,BALB/C和ICR小鼠次之。C57BL/6和对照组无显著性差异,提示对空弯菌抗原诱导的自身免疫应答不敏感。在遗传背景的制约下,性别也影响自身抗体的产生,有三种可能:(1)自身抗体升高主要见于雌性小鼠,如雌性KM小鼠被测的各种自身抗体均有升高;(2)雌性,雄性小鼠均有一种以上自身抗体升高,如SMMC/B和BALB/C小鼠;(3)雌、雄性小鼠的自身抗体均无升高,如C 57BL/6。本文结果表明,空弯菌苗诱导的自身抗体种类和水平,受小鼠的遗传背景和性别两个因素的相互制约和影响。

Five strains of mice (C57BL/6, BALB/c, ICR, KM and SMMC/B) were respectively orally immunized with CJ-S131 vaccine for 16 weeks, Autoantibodies against ss-DNA, ds-DNA, histones, extractable nuclear antigens (ENA)and thymocytes were detected by ELISA or CELIS(?)One or more kind (s) of autoantibody significantly raised in the serum of SMMC/B, KM, BALB/c and ICR mice, but not in C57BL/6 mice. The mean enzyme index (EI) of the autoantibodies of SMMC/B and KM mice was higher than that of ICR and BALB/c. The influence of the sex on the production of autoantibody induced by CJ-S131 vaccine seemed to be demonstrated on the basis of genetic background.It was characterized by (1) autoantibodies raising mainly in the female mice,e.g. in KM mice; (2) one kind of autoantibody or more raising in both male and female mice, as seen in SMMC/B and BALB/c mice; and (3) autoantibody no in male and female of C57BL/6 mice. The results revealed that the profile of autoimmune response induced by CJ-S131 vaccine was different in strains and sex of mice.