肝素锂血浆与血清样本进行甲状腺功能指标测定的对比分析

Lithium Heparin Plasma and Serum Samples were Analyzed for Determination of Thyroid Function Indexes

目的探讨肝素锂血浆行甲状腺功能指标测定的可行性。方法随机选取该院门诊患者和健康体检者43例。按常规方法分别采血至2个真空玻璃试管中各3 m L,其中一管不抗凝,另外一管为肝素锂抗凝。取血后充分混匀即刻离心分离血浆,不抗凝血清室温放置1 h后血清析出离心分离血清供检。于同一Uni Cel Dx I800 Access化学发光仪进行指标测定。结果肝素锂血浆与血清的甲状腺功能测定结果经比较,T3、FT3、FT4、TSH肝素锂血浆与血清结果差异无统计学意义(P>0.05),肝素锂血浆的T4与血清结果差异有统计学意义(P<0.01)。相关分析显示甲状腺功能测定结果肝素锂血浆与血清均具有高度正相关关系(r>0.700)。结论肝素锂血浆适合甲状腺功能指标测定,但肝素锂血浆的T4测定结果需建立回归方程将血浆值换算成血清值。

Objective To investigate the feasibility of heparin lithium plasma thyroid function indexes were detected. Methods 43 cases of outpatients of our hospital and health check-up random sampling. According to conventional methods of blood samples were collected to the 3ml 3 vacuum glass tube, a tube without anticoagulant, heparin lithium anticoagulant tube for another. Blood was taken immediately after mixing centrifugal separation of plasma samples for serum separation, centrifugal separation of serum at room temperature for 1 hour. After anti coagulation, in the same Uni Cel Dx I800 Access chemiluminescence analyzer was used to measure. Results Thyroid function in lithium heparin plasma and serum by comparison, no statistically significant T3, FT3, FT4,TSH in plasma and serum lithium heparin（P〉0.05）, the difference was statistically significant T4 in serum and heparin lithium plasma difference（P〈0.01）. Correlation analysis showed that the results of the thyroid function of heparin lithium plasma and serum were highly positive correlation（r 0.700）. Conclusion Lithium heparin plasma for thyroid function indexes, but lithium heparin plasma T4 determination results need to establish the regression equation of plasma values of serum converted value.