血液透析患者左心室肥厚与单核细胞趋化蛋白-1基因多态性关系研究

The study on relationship between Left Ventricular Hypertrophy and monocyte chemoattractant protein-1 gene polymorphism in hemodialysis patients

目的 观察血液透析患者左心室肥厚与单核细胞趋化蛋白-1（MCP-1）基因多态性的关系,探讨血液透析患者左心室肥厚的遗传易感因素.方法 选取血液透析患者142例,根据左心室重量指数结果分为肥厚组和无肥厚组,运用聚合酶链式反应限制性片段长度多态性技术（PC-RRFLP）检测血液透析患者MCP-1基因调节区-2518A/G基因多态性,分析左心室肥厚程度与MCP-1基因多态性的关系.结果 MCP-1-2518位点基因多态性在左心室肥厚组的分布：8例（8.3％）为AA基因型,50例（51.5％）为AG基因型,39例（40.2％）为GG基因型;无左心室肥厚组的分布为AA基因型7例（15.6％）,AG基因型28例（62.2％）,GG基因型10例（22.2％）;两组基因型间x2值为5.073,P值为0.079,无统计学差异;-2518A/G等位基因频率在左心室肥厚组的分布：A等位基因占34.0％,G等位基因占66.0％;在无肥厚组的分布为：A等位基因比例为46.7％,G等位基因比例为53.3％;两组间等位基因频率比较有统计学差异（x2=4.172,P=0.041）.肥厚组中MCP-1基因-2518位点G等位基因频率显著高于无肥厚组,差异具有统计学意义（P＜0.05）.结论 MCP-1-2518位点等位基因G患者更易发生左心室肥厚。

Objectives To observe the relationship between left vcntricular hypertrophy （LVH） and monocyte chemoattractant protein-1 （MCP-1） gene polymorphism and explore the genetic susceptibility factors of LVH in hemodialysis patients.Methods 142 patient cases of hemodialysis patients were included.Dividing the cases into LVH group and non-LVH group according to the LVMI.valuing the blood stasis of 142 cases,Measuring the MCP-1 gene regulatory region-2518 A/G gene polymorphisms differences in hemodialysis patients by using the polymerase chain reaction restriction fragment length polymorphism （PC-RRFLP）,the relationship between the degree of LVH and MCP-1gene polymorphism was analyzed.Results The distribution of the MCP-1-2518 gene polymorphism is 8 cases（8.3%） for the AA genotype,50 cases（51.5%） for the AG genotype,and 39 cases（40.2%） for the GG genotype in LVH group;the distribution of non-LVH group is the AA genotype 7 cases（15.6%）,AG genotype 28 cases（62.2%）,the GG genotypein10 cases（22.2%）;no significant difference of genotypes in two groups;Allelefrequency for A and G were 34.0% and 66.0% in the LVH group;Allelefrequency for A and G were 46.7% and53.3% in the non-LVH group;allele frequencies between the two groups were significantly different（P 〈 0.05）.There was a statistically significant differencc in the relationship between MCP-1 gene polymorphism and LVH （P 〈 0.05）,LVH group of MCP-1 gene-2518G allelefrequency was significantly higher than non-LVH group.Conclusions The MCP-1-2518 G allele was more likely to have left ventricular hypertrophy.