miR-181b-5p对卡波西肉瘤细胞系SLK细胞迁移、侵袭的影响

Effects of microRNA-181b-5p on Migration and Invasion of Human Kaposi's Sarcoma Cell Line SLK

目的通过脂质转染miR-181b-5p模拟物及抑制物到卡波西肉瘤细胞系SLK,研究miR-181b-5p对卡波西肉瘤细胞SLK细胞增殖、迁移、侵袭生物学功能的影响。方法应用脂质体对人卡波西肉瘤细胞株SLK进行转染,转染miR-181b-5p模拟物及抑制物后应用MTT测定细胞增殖曲线,应用流式细胞仪行检测细胞周期。利用Transwell小室试验后行苏木素染色观察细胞迁移、细胞侵袭生物学特性。结果 miR-181b-5p模拟物促进细胞增殖,促进S期的转变,加速细胞周期进程;miR-181b-5p抑制物抑制细胞增殖,诱导细胞发生G0/G1期阻滞,延缓细胞周期进程。Transwell小室迁移实验结果显示,与阴性对照组迁移实验下室面细胞数目151±11个相比,miR-181b-5p模拟物组184±9个,细胞数目明显数量增多,迁移能力均明显提高,差异有统计学意义(P<0.05)。Transwell小室侵袭实验结果显示,与阴性对照组侵袭实验下室面细胞数目35±6个相比,miR-181b-5p模拟物组48±5个,细胞数目明显数量增多,侵袭能力均明显提高(P<0.05)。结论 miR-181b-5p在SLK细胞中高表达,其对SLK细胞的增殖、侵袭和迁移能力可能存在正向调控作用,可能成为卡波西肉瘤的潜在治疗靶点。

Objective miR - 181b - 5p mimics and inhibitors were transfected into Kaposi＇s sarcoma cell line SLK through liposom- al transfeetion. To study the effect of miR -181b- 5p on SLK cell proliferation, migration and invasion biological activities. Methods Human Kaposi＇s sarcoma cell line SLK was transfected by liposomes. After miR - 181 b -5p mimic or inhibitor was transfected, SLK cell proliferation was assessed by MTT assay. Cell cycle of each group was determined by flow cytometry after 48 hours after transfection. After miR - 181 b - 5p mimic or inhibitor was transfected, cell migration and invasion was determined by transwell assay followed by hematoxylin staining after 24 hours. Results miR - 181b -5p mimic promoted cell proliferation,promoted transformation and accelerated the process of cell cycle, miR- 181b- 5p inhibitor inhibited cell proliferation, induced G0/G1 phase retardation, delayed the process of cell cycle. Transwell migration assay results showed that number of migrated cell in the group treated with miR -1181b -5p mimics was 184 ±9, sig- nificantly higher than that of control group 151 ＋ 11, suggesting that miR - 181b -5p mimics significantly increased cell migration ability （ P 〈 0.05 ）. Transwell invasion assay showed that cell number of miR - 181b -5p mimics group 48±5 significantly increased compared to negative control group 35 ± 6 suggesting increased invasiveness （P 〈 0.05 ）. Conclusion miR -181 b -5p may positively regulate the proliferation, invasion and migration of the SLK cell. miR- 181 b- 5p may be a potential treatment taget for Kaposi＇s sarcoma.