酶解法检测牛奶中酵母β-葡聚糖含量的方法

Enzymolysis method for quantification of yeast β-glucan in milk

详细描述了利用蛋白酶解法和葡聚糖酶解法检测酵母β-葡聚糖的方法。并对该方法进行了优化,最后验证了测定方法的精密度,回收率及日间重复性实验。实验结果显示,该检测方法测得的酵母β-葡聚糖质量分数的精密度RSD为1.5%,平均回收率均在87%~98%之间,日间重复性稳定。此方法适用于作为牛奶中酵母β-葡聚糖特别是[（1-3）（1-6）-β-glucan）]质量分数的测定方法。

The content of yeast β-glucan added in milk was quantified by using protease hydrolysis with glucanase hydrolysis method. The ：ondition of this method was also improved. The precision and recovery of the test was investigated. The results showed that the RSD value 3fprecision test was 1.5%. The average recovery ranged from 87% to 98%, and the intra-day and inter-day assay was stable. In conclusion, the developed method allows yeast β-glucan quantification （specifically for mixed-linkage （1-3）（1-6）-β-glucan）to be conducted in milk.