摘要
为了给脐带间充质干细胞(umbilical cord mesenchymal stem cells,UCMSCs)的临床应用提供依据,以鸭胚UCMSCs为研究对象,采用胶原酶Ⅳ消化法分离脐带间充质干细胞,用表面标记分子CD29,CD44,CD71,CD73对纯化细胞进行鉴定,建立了鸭胚UCMSCs的体外培养体系。结果表明,UCMSCs能够诱导分化为脂肪细胞、成骨细胞,证明了鸭胚UCMSCs的多能性。
In order to provide experimental evidence for the clinical application of umbilical cord mesenchymal stem cells（ UCMSCs） in the future,UCMSCs of duck were isolated in the present study by collagenase Ⅳ digesting method and were identified by adhesion molecules,such as CD29,CD44,CD71,CD73. A culture system of duck embryo umbilical cord derived mesenchymal stem cells was established. It was shown that UCMSCs isolated and cultured by this method could be differentiated into adipocytes and osteoblasts,which proved their multi- differentiation ability.
出处
《浙江农业学报》
CSCD
北大核心
2015年第11期1903-1909,共7页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(31472099)
中国农业科学院科技创新工程项目(cxgc-ias-01)
关键词
鸭胚
脐带间充质干细胞
生物学特性
诱导分化
duck embryo
umbilical cord mesenchymal stem cells（UCMSCs）
biological characteristic
differentiation
