铜胁迫对铜绿微囊藻叶绿素荧光的影响

Effects of Cu^(2+) Stress on Chlorophyll Fluorescence of Microcystis aeruginosa

[目的]为了解硫酸铜对铜绿微囊藻的毒害效果。[方法]利用叶绿素荧光技术,研究不同浓度的Cu^(2+)胁迫对铜绿微囊藻光合作用的影响。[结果]铜胁迫对铜绿微囊藻藻细胞的光合具有明显抑制作用。随着Cu2+浓度的增加,微囊藻受到不同程度的抑制。随着铜离子浓度的增加,最大光化学效率(FV/FM)呈现下降趋势,光系统II(PSII)的潜在活性(F_V/F_0)和光合电子传递能力(ETO/RC)也呈下降趋势,而单位有活性的反应中心耗散的光能(DIO/RC)、单位反应中心吸收的光能(ABS/RC)以及热耗散量子比率(ΦD0)则逐渐上升,以减少铜胁迫对PSII的损害。[结论]在利用硫酸铜控制藻类生长时,应选择适宜的铜离子浓度,既可以达到杀藻的目的,又可以减少环境污染。

[ Objective ] The research aimed to explor the toxic influence of copper sulfate on Microcystis aeruginosa. [ Method ] The chlorophyll fluorescence technology was used to explore the response of its photosynthesis to copper. [ Result] Copper threat could suppress the pho- tosynthesis of algae cell, with the increase of Cu2＋ content, Fv/Fu, Fv/Fo and ETO/RC decreased as well. At the same time, DI0/RC, ABS/RC, and ФD0 rose gradually to reduce the damage to PSII caused by copper. [ Conclusion ] When we utilized copper sulfate to control the growth of algae, we should arranged appropriate copper ion content to balance the control of algae and environment protection.