烟草内生真菌及其提取物的抗细菌活性(英文)

Endophytic Fungi from Nicotiana tabacum L. and Their Antibacterial Activity

从健康烟草的根和茎中分离到38株内生真菌,对23株代表性内生真菌菌株进行了形态观察和ITS-r DNA分析,共鉴定出18个属,其中Acremonium(菌株Nitaf01和Nitaf02)、Fusarium(菌株Nitaf07、Nitaf08和Nitaf09)、Penicillium(菌株Nitaf18和Nitaf20)和Plectosphaerella(菌株Nitaf11和Nitaf12)为主要属,有13个属(Acremonium、Cladosporium、Clonostachys、Ilyonectria、Mortierella、Myriodontium、Petriella、Plectosphaerella、Podospora、Purpureocillium、Rhizopycnis、Stephanonectria和Thielavia)的真菌为首次从烟草中分离得到。5株内生真菌(Colletotrichum sp.Nitaf05、Fusarium sp.Nitaf07、Purpureocillium sp.Nitaf13、Penicillium sp.Nitaf20和Rhizopycnis sp.Nitaf22)的菌丝和发酵液提取物均表现出较强的抗细菌活性。结果表明烟草存在丰富的内生真菌,这些内生真菌具有生产抗菌活性成分和作为生防菌剂应用的潜力。

A total of 38 endophytie fungal isolates were separated from the healthy roots and stems of tobacco （Nicotiana tabacum） ,and 23 representative fungal isolates were selected for further identification by morphology features and ITS- rDNA analysis. Among 18 genera obtained,Acremonium （ isolates Nitaf01 and Nitaf02）, Fusarium （ isolates Nitaf07, Nitaf08 and Nitaf09） ,Plectosphaerella （isolates Nitafll and Nitaf12） and Penicillium （isolates Nitaf18 and Nitat20） were dominant genera. 13 genera, namely Acremo nium , Cladosporium , Clonostach ys , llyonectria , Mortierella , Myriodontium , Pet- riella , PlectosphaereUa , Podospora , Purpureocillium , Rhizopycnis , Stephanonectria and Thielavia were isolated from N. tabacum for the first time. Both mycelia and filtrate extracts of five fungal isolates （ Colletotrichum sp. Nitaf05, Fusarium sp. Nitaf07 ,Purpureocillium sp. Nitafl3 ,Penicillium sp. Nitat20 and Rhizopycnis sp. Nitat22） showed Strong antibacterial activity. The results demonstrated a diversity of the endophytic fungi in N. tabacum. These endophytic fungi had potential to produce antimicrobial compounds as well as to be used as the biocontrol agents.