UPLC-MS法测定羊耳菊中6种成分的含量

Simultaneous Determination of Six Components in Inula cappa by UPLC-MS

建立了UPLC-MS/MS同时测定羊耳菊药材中东莨菪苷、1,3-O-二咖啡酰基奎宁酸、木犀草苷、3,4-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、4,5-O-二咖啡酰基奎宁酸6个成分含量的方法。采用Waters BEH C18（2.1 mm×100 mm,1.7μm）色谱柱,用0.1%甲酸乙腈溶液-0.1%甲酸水溶液作流动相,梯度洗脱,电喷雾电离源（ESI）,以多反应离子监测（MRM）检测。6种成分分别在0.058~42.000、0.178~129.630、0.087~63.400、0.288~69.930、0.172~125.500、0.184~134.130μg/m L浓度范围线性良好,平均回收率为97.84%~105.67%（RSD〈1%）。稳定性,重复性及精密度良好,本方法能简便、快捷、有效的测定羊耳菊药材中多种成分含量,为羊耳菊药材的全面质量控制提供了新的方法。

To establish a UPLC-MS/MS method for the determination of seopolin, 1,3-di-O-caffeoylqalnic acid, luteolin- 7-O-β-D-glucoside ,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid in Inula cappa. Using Waters BEH C18 colunm （2.1 mm × 100 mm, 1.7 μm） with a gradient solvent system of 0. 1% formic acid in acetonitrile-0. 1% formic acid in water as mobile phases. Multiple reactions monitoring （MRM） mode was used as detection mode with electro-spray ionization （ESI） source. Good linearities were achieved in the ranges of 0. 058- 42. 000,0. 178-129.630,0.087-63.400,0. 288-69.930,0. 172 - 125.500 and 0. 184-134. 130 μg/mL for the 6 compounds, respectively. The average recoveries were 97.84% -105.67% （ RSD 〈 1% ）. Precision, repeatability and stability were good. The developed method was simple, rapid and effective for the determination of the components in L cappa. It provided a new method for the quality control of I. cappa.