摘要
本论文采用Box-Behnken响应曲面设计法,优化超临界CO2萃取松茸精油的工艺,并采用GC-MS分析该松茸精油的化学成分。优化的最佳工艺为:萃取压力32.46 MPa,萃取46.01℃,89.15%的乙醇作为夹带剂。同时,GC-MS的分析结果显示松茸精油的主要成分为9,12-十八碳二烯酸、麦角固醇和9,12-十八碳二烯酸甘油酯。其中,9,12-十八碳二烯酸和麦角固醇为松茸精油中抑制黑素合成的主要成分。
In this study,a supercritical carbon dioxide extraction method was employed to extract the essential oil from Tricholoma matsutake. The extraction conditions were optimized by response surface methodology （RSM）. Furthermore, the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry （GC-MS）. The op- timal conditions to obtain the highest yield of essential oil were determined as ：32.46 MPa pressure,46.01 ℃ tempera- ture and 89.15 vol. % ethanol. Furthermore,9,12-octadecadienoic acid, ergosterol and 2,3-dlhydroxypropyl 9,12-octa- decadienoate were the major constituents presented in T. matsutake. Among them,9,12-octadecadienoic acid and ergos- terol can inhibit melanin synthesis.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第11期1943-1949,共7页
Natural Product Research and Development
基金
National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(2011BAD23B00)
China Cosmetic Collaborative and Innovation Center Grant,BTBU(19008001060)
关键词
松茸
精油
超临界萃取
响应面
成分分析
Tricholoma matsutake
essential oil
supercritieal fluid extraction
response surface methodology
chemical analysis
