摘要
基于1H NMR的代谢组学方法对灌胃给药赭石的成年Wistar大鼠血清进行分析,运用主成分分析模式识别方法对所得数据进行处理,并对给药大鼠血清进行生化指标检测.研究结果表明,大鼠体内β-羟异丁酸、乙酸、丙酮、胆碱、甘油磷脂酰胆碱、葡萄糖、乳酸、低密度脂蛋白、极低密度脂蛋白和脂质等内源性代谢物浓度发生明显变化,可作为赭石的特征代谢物.2 g/kg和5 g/kg体重剂量赭石使大鼠机体产生大量活性氧化物(ROS),造成过氧化损伤,导致能量代谢和糖代谢紊乱,糖酵解反应增强,并且对肝功能造成了影响.
Wistar rats were intragastrically administered with different doses（0, 2, 5 g/kg body weight） of haematitum. 1H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of β-hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species（ROS） and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.
出处
《波谱学杂志》
CAS
CSCD
北大核心
2015年第4期628-636,共9页
Chinese Journal of Magnetic Resonance
基金
国家自然科学基金资助项目(20975097
21305134)
关键词
核磁共振(NMR)
代谢组学
主成分分析
赭石
nuclear magnetic resonance
metabonomics
principal component analysis
haematitum
