清火片含量测定的HPLC方法研究

HPLC Method for the Determination of Content of Qinghuo Pian

建立了清火片含量测定的方法。对大黄药材中大黄素和大黄酚提取方法进行实验对比研究,利用HPLC法进行含量测定。结果表明：大黄素浓度在2.56~160μg/m L范围内吸收度成良好线性关系（R2=0.9999）,平均回收率为107.1%,RSD为1.40%;大黄酚浓度在1.72~34.4μg/m L范围内吸收度成良好线性关系（R2=0.9997）,平均回收率为100.91%,RSD为0.82%。HPLC法进行含量测定方法简便、准确可靠,可用于清火片的含量测定。

Content determination of Qinghuo Pian was established. Emodin and rhubarb phenol extraction in rhubarb medicinal materials were discussed, content using HPLC method for determinate. Results showed that concentration of emodin was up to 2. 56~160 μg/mL with a good linear relationship with absorbance （ R2=0. 9999 ） , the average recovery was 107. 1%, RSD was 1. 40%. Concentration of rhubarb phenol was up to 1. 72 to 34. 4 μg/mL with a good linear relationship with absorbance （ R2 = 0. 9997 ） , the average recovery was 100. 91%, RSD was 0. 82%. HPLC method for content determination method is simple, accurate, reliable and can be used for content determination of Qinghuo Pian.