不同提取方法对黄连中小檗碱含量及抑菌性能的影响

Influence of different extracting method on the content of berberine in rhizoma coptis and antifungal performance

研究不同提取方法对黄连中小檗碱含量的影响和相应提取物的抑菌性能。选用对真菌有良好抑制作用的黄连作为提取药材,利用水和95%乙醇作为提取溶剂,采用微波和超声处理作为辅助提取手段,以盐酸小檗碱提取率为指标,采用高效液相色谱法测定含量;以木材白腐菌和褐腐菌作为试验菌种验证黄连不同提取物的抑菌效果。结果显示,黄连粉末加以8倍量95%乙醇,浸泡12h,超声600W、20k Hz脉冲处理30min,回流4h所得小檗碱含量最大为19.933g/100g;各种提取方法所得提取物均有良好抑菌效果,但以醇提超声处理效果最佳,且各种提取物对褐腐菌抑制效果较白腐菌明显。

To explore the effect of different extracting methods on the content of berberine hydrochloride in Rhizoma Coptis and the antifungal effect of the corresponding extracts, we choose Rhizoma Coptis as test materials and use water and 95% ethanol as extraction solvent, by microwave and ultrasonic treatment as auxiliary extraction method,and use extraction rate of berberine hydrochloride as index and use high performance liquid chromatography to determine the content. We use wood white-rot fungi and brown-rot fungi as the research object to verify the antifungal effect of different extractive of Rhizoma Coptis. The results showed that the powder of Rhizoma Coptis was mixeded with 8 times amount of 95% ethanol,soak 12 h,ultrasonic 600 w, 20 KHz pulse processing 30 min, reflux 4h, the maximum content of berberine hydrochloride reach to 19.933g/100 g. And each of six kinds of extracts has good antifungal effect, but the ethanoland ultrasonic processing effect is the best and the antifungal effect of all kinds of extracts on brown-rot fungus is better than that of white-rot fungus.