胶质瘤细胞自发分泌免疫抑制因子的研究

Study of JCBI spontaneously producing a suppressor factor

体外研究JCBI人星形细胞瘤细胞系发现,这株细胞可自发分泌一种免疫抑制因子,对小鼠胸腺细胞ConA增殖反应和T淋巴母细胞对IL-2依赖的增殖反应均有抑制作用。理化性质分析表明,这种抑制因子为分子量接近10Kd的不耐热因子。这一发现揭示了胶质瘤患者免疫功能缺陷和胶质瘤的内在联系。

JCBI is a glioma eell line established in our department. JCBI cells spontaneously produce a factor inhibiting blastogenic responses of mouse thymocytes and the IL-2 dependent T cell growth of mouse spleen lymphoblast, without inhibiting spontaneous proliferation of mouse thymocytes. The inhibiting activity is high after 72 hours of passage. Physicochemical characterization shows that the factor is nondialyzable. partially inactivated at 37℃ 48 hours and almost inactivated at 56℃ 30 min. But the factor is stable at 4℃ for 2 weeks and in freeze-thawing. The molecular weight of the factor is approximately 10 kd as estimated by gel filtration. The factor may contribute to the impaired immunosurveillance and to the cellular immunodeficiency state in the patients.