人工饲养树鼩皮肤真菌携带的调查

The survey of dermatophyte in artificial feeding of tree shrews

目的调查人工饲养条件下树鼩皮肤真菌携带情况,为实验树鼩的微生物质量控制提供依据。方法刮取79只树鼩皮肤表皮碎屑,包括野生来源的51只和人工繁殖的F1代28只,采用沙氏青霉素瓶斜面法和沙氏平皿培养法分别进行培养,对长出的每株真菌进行取样涂片染色,显微镜镜检观察真菌形态,用酚氯仿法提取真菌DNA,PCR扩增ITS基因,测序后经BLAST进行核酸同源性比对,进一步鉴定分析。结果野生来源树鼩有9份样长出真菌,F1代有3份样长出真菌菌落,携带率分别15.69%和10.71%。所有真菌DNA最后进行BLAST比对鉴定后发现为7个种属的真菌,但均无致病性。结论人工饲养的树鼩种群中皮肤真菌携带率较低,并且随繁殖代次增加而下降,未检测到皮肤病原真菌。

Objective To investigate the pathogenic dermal fungi in tree shrews from the center of germplasm resources,to provide the basic data for the experimental tree shrews ＇microbiological quality control. Methods 79 samples obtained from tree shrews were inoculated on Sabouraud＇s platiculture and slant cultivation,51 of which are from the wild origin and 28 from artificial feeding F1 generation,and they were subjected to microscope observation. Then extracted DNA with traditional methods. Amplify the ITS gene by PCR. Finally sequencing by blast of nucleotide homology analysis. Results 9 samples of the wild tree shrews turned into growing fungus,and so as 3 of the F1 generation. The carrying rate are 15. 69% and 10. 71% respectively. All fungi were found to be non-pathogenic followed BLAST.Conclusion The carrying rate of dermatophyte in artificial feeding of tree shrews is very low,and which decreased with the increasing breeding generation. Pathogenic dermal fungi has not been found.