丁硫克百威残留对土壤酶活及微生物的影响

The Influence of Carbosulfan Residue on Soil Enzymes Activity and Microbial

采用室内培养法研究了丁硫克百威2、8、20、50 mg/kg 4个浓度处理对土壤微生物数量、酶活性以及微生物群落功能多样性的影响。结果表明,丁硫克百威对土壤放线菌种群有明显抑制作用,对细菌和真菌影响较小。2、8、20 mg/kg浓度处理对土壤蔗糖酶有促进作用,50 mg/kg浓度处理抑制蔗糖酶活性;各浓度处理对脲酶和碱性磷酸酶均表现抑制作用;2 mg/kg浓度处理对过氧化氢酶表现为"抑制-促进-抑制"的变化趋势,而8、20、50 mg/kg浓度处理对过氧化氢酶有很强抑制作用;处理7 d后低浓度丁硫克百威的AWCD值低于对照水平,而高浓度的AWCD值高于对照水平,14 d后基本恢复对照水平;各处理组的Shannon指数在7 d后与对照有差异,14 d后基本恢复至对照水平;Simpson指数在7 d和14 d后与对照基本一致;50 mg/kg浓度处理的Mc Intosh指数高于对照。

The effect of 4 different concentrations（2,8,20 and mg/kg） of Carbosulfan on soil microbial quantity,activity and functional diversity of communities were studied by incubation experiments. The results showed that actinomycetes in soil was significantly inhibited, bacteria and fungi in soil was slightly inhibited by carbosulfan residue. The activity of sucrose was promoted by 2,8 and 20 mg / kg concentration of carbosulfan residue, but it was restrained by 50 mg / kg concentration.The activities of urease and alkaline phosphatase were inhibited by all the concentration of carbosulfan residue. The tendency of catalase activity was ＂suppression-promotion-suppression＂, which was treated by 2 mg / kg concentration of carbosulfan residue,however,it was greatly suppressed by 8,20,50 mg / kg concentration. After 7 d treatment,the AWCD value of low carbosulfan residual concentrations was lower than CK,but the AWCD value of high concentrations was higher than CK.After 14 d it returned to CK level. The Shannon indexes for each treatment group were different from CK at 7 d, and they went back to CK level at 14 d. The Simpson indexes were always same as CK at 7 d and 14 d. Mc Intosh value of 50 mg / kg concentration was higher than CK.