EAE小鼠胸腺萎缩与疾病严重程度关系的初步探究

Preliminary inquiry EAE mouse atrophy of thymus relationship with disease severity

目的:研究EAE小鼠胸腺萎缩与疾病严重程度的关系。方法:MOG35-55肽免疫C57BL/6小鼠诱导EAE,分析EAE小鼠疾病严重程度与胸腺萎缩的关系,流式细胞分析胸腺CD4+CD8+双阳性细胞、CD4+CD8-、CD4-CD8+单阳性细胞与疾病严重程度的关系。结果:尾部无力小鼠胸腺淋巴细胞数为(20.25±3.49)×106个,双后肢无力小鼠胸腺淋巴细胞数为(4.93±0.85)×106个,双后肢瘫痪小鼠胸腺淋巴细胞数为(1.8±0.19)×106个,四肢完全瘫痪小鼠胸腺淋巴细胞数为(0.52±0.07)×106个,组间差异有统计学意义(P<0.05);EAE疾病越重,CD4+CD8+双阳性细胞在胸腺细胞中的比例越低,CD4+CD8-、CD4-CD8+单阳性细胞比例越高,不同疾病组间差异有统计学意义(P<0.05)。结论:EAE小鼠疾病严重程度与胸腺萎缩程度关系密切,EAE小鼠中活化T细胞的定向迁移可能导致胸腺萎缩。

Objective： To study the relationship between atrophy of the thymus and disease severity in EAE. Methods：MOG35-55 peptide induced EAE in C57 BL /6 mice and analyzed the relationship between the severity of EAE and thymic atrophy,Flow cytometry analysis was used to evaluate thymic CD4^＋CD8＋DP cells,CD4^＋CD8^-,CD4^-CD8^＋SP cells in relation to the severity of the disease. Results： The number of thymocytes in mice with decreased tail tone was（ 20. 25 ± 3. 49） ×10^6,hindlimb weakness（ 4. 93 ±0. 85） ×10^6,complete hindlimb paralysis（ 1. 8 ± 0. 19） ×10^6,and forelimb and hindlimb paralysis（ 0. 52 ± 0. 07） ×10^6,there were statistically significant differences between groups（ P〈0. 05）. As the disease progresses,CD4^＋CD8^＋DP cells ratio decreased,CD4^＋CD8^-,CD4^-CD8^＋SP cell ratio increased,different disease groups was statistically significant difference（ P〈0. 05）. Conclusion： The atrophy of thymus was closely related to the severity of EAE. Migration of activated T cells in EAE may cause atrophy of thymus.