三种不同脊髓机械性损伤对大鼠继发性神经细胞凋亡的影响研究

Different spinal cord damage on apoptosis of rat secondary impact study

目的:探讨脊髓挫伤、脊髓横断及持续性占位损伤对大鼠继发性神经细胞凋亡的影响作用。方法:选取健康雄性Wistar大鼠66只,进行编号后采用随机数字表法分为A(18只,脊髓挫伤)、B(18只,脊髓横断)、C(18只,持续性占位)、D(6只、假手术组)、E(6只、对照组)五组,分别观察伤后1、4、7d各组大鼠的神经细胞凋亡指数、脊髓组织中Bcl-2、Bax、caspase-3蛋白的表达情况。结果:A、B、C三组大鼠在造模后第1天均出现脊髓灰质和白质染色阳性标记,且三组大鼠的灰质、白质神经细胞凋亡指数差异具有统计学意义(P<0.05);在造模后第4、7天三组大鼠的灰质、白质区域脊髓凋亡细胞指数均呈现出增高趋势(P<0.05);在造模后第1、4、7天C组大鼠的脊髓灰质、白质区域凋亡细胞指数显著高于A组、B组,B组显著高于A组且差异均具有统计学意义(P<0.05)。造模后第1、4、7天A、B、C、D、E组五组大鼠的Bcl-2、Bax、caspase-3蛋白表达差异均具有统计学意义(P<0.05),造模后第1、4、7天A、B、C三组大鼠的Bcl-2、Bax、caspase-3蛋白表达显著高于D组和E组(P<0.05)。结论:大鼠脊髓损伤后神经细胞继发性凋亡显著,严重程度与损伤类型有关。

Objective： To study the spinal cord injury,spinal cord transection and persistent placeholder damage on the influence of secondary neural cell apoptosis in rats. Methods： Select 60 healthy male Wistar rats,numbered after using the random number table method is divided into A（ 18,spinal cord contusion）,B（ 18,spinal cord transection）,C（ 18,continuous placeholder）,D（ 6,control）,E（ 6,the control group only） groups of five,were observed at the 1,4,7 D after 5 group of rats nerve cell apoptosis index,spinal cord tissue Bcl-2,the expression of Bax,caspase 3 protein. Results： A,B,C three groups of rats after building 1 d are gray and white matter positive markers,and the gray matter and white matter of three groups of rats nerve cell apoptosis index differences statistically significant（ P〈0. 05）; 4 d,7 d after building gray matter and white matter of three groups of rats tend to place increased apoptotic cells in the spinal cord index（ P〈0. 05）; in building 1,4,7 d group C after rat spinal cord grey matter and white matter of apoptotic cell index was significantly higher than that of group A and group B,group B were significantly higher in group A and the differences were statistically significant（ P 〈0. 05）. 1,4,7 d after building A,B,C,D,E five group rats the Bcl-2,Bax,caspase-3 protein expression differences were statistically significant（ P〈0. 05）,1,4,7 d after building A,B,C the Bcl-2 of three groups of rats,Bax,caspase-3 protein expression was significantly higher than that of group D and group E（ P〈0. 05）. Conclusion： Secondary rats after spinal cord injury of nerve cells apoptosis,apoptosis time,severity,and damage type and severity.