亥茅酚苷对脂多糖诱导巨噬细胞一氧化氮和白介素6表达的影响

Effects of Sec-O-Glucosylhamaudol on expressions of nitric oxide and interleukin-6 in Lipopolysaccharide stimulated murine macrophage

目的:探讨亥茅酚苷对脂多糖(LPS)诱导的小鼠巨噬细胞株RAW264.7炎症介质及细胞因子(NO、IL-6)释放的影响。方法:应用LPS(1μg/ml)刺激RAW264.7细胞,采用不同浓度亥茅酚苷(20、40、80μg/ml)干预,Griess试剂法测定NO释放量;酶联免疫吸附试验(ELISA)测定IL-6释放,用免疫印迹法(Western blot)检测细胞一氧化氮合酶(i NOS)的表达,实时荧光定量反转录聚合酶链反应(RT-PCR)技术分析i NOS、IL-6 mRNA的表达。结果:亥茅酚苷各剂量组(20、40、80μg/ml)均能抑制LPS诱导的RAW264.7细胞NO、IL-6的释放(P<0.01),并下调i NOS蛋白、i NOS mRNA、IL-6 mRNA的表达。结论:亥茅酚苷对LPS诱导的巨噬细胞NO、IL-6释放和i NOS表达有抑制作用,具有一定的抗炎活性。

Objective： To investigate the effect of Sec-O-Glucosylhamaudol on inflammatory mediator and cytokine（ NO and IL-6） production in Lipopolysaccharide（ LPS） stimulated murine macrophage（ RAW264. 7）. Methods： Macrophages were induced with LPS,and incubated with different concentrations of Sec-O-Glucosylhamaudol（ 20,40,80 μg/ml）,the quantity of NO production was measured by Griess reagent; the IL-6 production were measured by enzyme linked immunosorbent assay（ ELISA）,the expression of nitric oxide synthase（ iNOS） in cells were detected by Western blot; the expression of iNOS and IL-6 mRNA were analyzed by real-time PCR. Results： Each concentrations of Sec-O-Glucosylhamaudol（ 20,40,80 μg/ml） inhibited the production of NO and IL-6 in LPSstimulated RAW264. 7 cells（ P〈0. 01）. This compound also reduced the mRNA expression of iNOS and IL-6. Conclusion： Sec-O-Glucosylhamaudol exhibited anti-inflammatory activity by inhibited the NO and IL-6 production in LPS stimulated RAW264. 7 cells.