白虎汤对全身炎症反应综合征大鼠肺组织损伤的影响及机制探讨

Influence of Baihu decoction on lung injury of rats with systemic inflammatory response syndrome

目的观察应用白虎汤后全身炎症反应综合征(SIRS)大鼠血浆TNF-α水平及肺组织的变化,探讨白虎汤对发生SIRS后肺组织的影响及机制。方法将60只大鼠随机分为对照组、模型组、中药组及西药组,每组各15只。模型组、中药组及西药组均行腹腔注射大肠杆菌脂多糖20 mg/kg制备SIRS模型。对照组不造模。模型组、西药组、中药组于造模后分别予生理盐水灌胃、氢化可的松琥珀酸钠静脉注射及白虎汤灌胃,共3 d。造模后第4日,各组大鼠腹主动脉采血,采用ELISA法检测血浆TNF-α水平。随后处死大鼠取肺组织,肉眼及HE染色后光镜下观察肺组织病理表现。结果对照组、模型组、西药组、中药组血浆TNF-α水平分别为(64.05±7.72)、(74.25±8.67)、(62.63±7.01)、(58.78±9.68)pg/m L,模型组TNF-α水平均高于对照组、中药组及西药组(P均<0.05)。光镜下,对照组肺组织结构完整,肺泡腔清晰,肺泡隔无水肿、炎细胞浸润等改变;模型组肺间质充血,伴炎细胞浸润,肺泡隔明显增厚;中药组、西药组肺组织病理改变均较模型组减轻,且中药组改善程度更好。结论白虎汤对SIRS大鼠肺组织具有保护作用,其机制可能与减少TNF-α的释放有关。

Objective To observe the effect of Baihu decoction on the changes of TNF-ɑ and lung tissues of rats with systemic inflammatory response syndrome（ SIRS）,and to investigate the influence and mechanism of Baihu decoction on the lung tissues of the SIRS model rats. Methods Sixty rats were randomly divided into the control group,model group,and Chinese medicine Baihu decoction treatment group（ Chinese medicine group） and Western medicine hydrocortisone sodium succinate treatment group（ Western medicine group）,each group had fifteen rats. The SIRS model rats were made in the model group,Chinese medicine and Western medicine group by escherichia coli lipopolysaccharide（ 20 mg / kg）. After modeling,the rats of the model group,Western medicine group and the Chinese medicine group were respectively treated with normal saline by gavage,hydrocortisone sodium succinate and Baihu decoction by vein for 3 days. On the fourth day,the rat abdominal aortic blood of each group was acquired,and the plasma TNF-ɑ level was measured by enzyme-linked immunosorbent assay（ ELISA）,then we killed the rats and took out the lung tissues. The pathological changes of the lung tissues were observed by the naked eyes and light microscopy after HE staining. Results The levels of TNF-ɑ of the control group,model group,and Chinese medicine group and Western medicine group were respectively（ 64. 05 ± 7. 72）,（ 74. 25± 8. 67）,（ 62. 63 ± 7. 01） and（ 58. 78 ± 9. 68） pg / m L; the TNF-ɑ level of the model group was higher than that of the control group（ P〈0. 05）,the TNF-ɑ levels of the Chinese medicine group and Western medicine group were lower than that of the model group（ P〈0. 05）. Under the light microscopy,the lung tissues of the control group had a structural integrity,clear alveolar space and alveolar septa without edema,no inflammatory cell infiltration and other changes; the model group had interstitial pulmonary congestion complicated with inflammatory cell infiltration,and thickened alveolar septa.The histopathological changes of the Chinese medicine and Western medicine group were significantly lighter than that of the control group,and the histopathological change of the Chinese medicine group was better than that of the Western medicine group. Conclusion Baihu decoction has protective effect on the lung tissues of SIRS rats,and the mechanism may be related to decrease of TNF-α release.