胞内劳森氏菌lsaA基因克隆与序列分析

Cloning and sequence analysis of lsa A gene of Lawsonia intracellularis

目的:克隆并分析胞内劳森氏菌(Lawsonia intracellularis,LI)lsa A基因,为研究该基因提供基础。方法:根据已发表的LI基因组序列,设计2条引物,提取猪回肠黏膜的LI基因组,并用PCR技术扩增lsa A基因,将其克隆、测序,进行序列同源性、蛋白抗原性预测等分析。结果:扩增出与702 bp引物相符的基因片段,与Gen Bank的序列同源性为98.4%,该基因编码的蛋白预计具有良好的抗原性。结论:胞内劳森菌las A基因的成功克隆为增生性肠炎的防控提供研究基础。

Objective： Cloning and sequence analysis of lsa A gene of Lawsonia intracellularis to lay the foundation for further study of the gene. Methods： 2 primers were designed to amplify Lawsonia intracellularis lsa A gene by PCR. Cloned and sequenced, the nucleotide sequence homology of this gene and protein antigenicity prediction analysis were carried out. Results： 702 bp PCR product in length were amplified, which have the sequence homology of 98.4% with that of published lsa A gene in Genbank. The protein lsa A encoded by lsa A gene is predicted to have good antigenicity by using DNAStar soft. Conclusion： The successful cloning of Lawsonia intracellularis lsa A gene lay the foundation for the prevention and control of proliferative enteritis.