13μs,200ns和2ns脉冲电场对酵母菌杀灭效果分析

Analysis of inactivation saccharomyces cerevisiae by 13μs,200ns and 2ns pulsed electric fields

研究了脉冲宽度对高压脉冲下细胞膜和细胞器膜跨膜电压的影响及细胞膜能量的变化,以及13μs,200ns和2ns脉冲对酿酒酵母(CICC31180)杀灭作用时脉冲数的影响。结果表明,随着脉冲数增加,13μs,200ns和2ns脉冲对啤酒酵母的灭杀效果均增强。在13μs脉宽,14kV电压和50个脉冲数下,酵母达到最小存活率-4.3个对数;在200ns脉宽,40kV电压和500个脉冲数下,酵母达到存活率-2.6个对数;在2ns脉宽,200kV电压和500个脉冲数下,酵母达到存活率-2.8个对数。三种脉冲对酵母菌杀灭的存活率对数与脉冲数的关系表现不是线性的,脉冲数增加到一定程度,存活率下降变缓。在同等灭杀效果-2.0个对数下,2ns脉冲比13μs脉冲在同轴电极中损耗的能量更少。

The effect of the pulse width change is studied on the membrane voltage and energy of cell membranes and organ-elles membrane under the high voltage pulse electric field. The sterilization effect of saccharomyces cerevisiae （CICC31180） chan-ges with the quantity of the 13 μs, 200 ns or 2 ns pulses. The results showed that with the increase of the quantity of pulses, thesterilization effect was enhanced. The yeast concentration decreased by 4.3 orders of magnitude after 50, 13 μs width, 14 kV pul-ses treatment while by 2.8 orders of magnitude after 500, 13 ns width 200 kV pulses treatment, by 2.6 orders of magnitude after500, 13 ns width 200 kV pulses treatment. The survival rate logarithmic relation with the pulse number was not linear. The sur-vival rate logarithmic decreased slowly when the pulse number increased to a higher value. Under the same survival rate logarith-mic decrease, the loss of energy in coaxial electrode was lower in 2 ns pulses than 13μs or 200 ns pulses.