期刊文献+

哮喘相关的miR-201-3p靶基因的预测及生物信息学分析 被引量:1

Prediction and bioinformatics analysis of the targets of miR-201-3p related to asthma
下载PDF
导出
摘要 目的:预测并分析miR-201-3p的靶基因。方法:利用miRanda、miRDB、miRWalk和Targetscan等4个数据库在线预测miR-201-3p的靶基因,对获得的靶基因用Cytoscape软件中的Bi NGO插件进行Gene Ontology(GO)分类及KEGG数据库的信号转导通路富集分析。结果:共预测获得1082个靶基因。经GO分析这些靶基因后,共得到涉及245条生物学进程,主要包括细胞进程、生物学调节和生物学进程调节等;分子功能包括蛋白质异源二聚体活化和I-SMAD蛋白结合等。KEGG富集分析发现这些靶基因主要参与MAPK信号通路、T细胞受体信号通路、Wnt信号通路、趋化因子信号通路等与炎症有关的信号通路。结论:成功预测了miR-201-3p的靶基因,部分靶基因可能参与了哮喘气道炎症和气道重塑。 Objective: To predict and analyze the molecular targets of miR-201-3p. Methods: Target gene miR-201-3p was predicted through the online databases of miRanda,miRDB,miRWalk and Targetscan. Then the predicted targets were further analyzed by Bi NGO plugin for Gene Ontology( GO) and database of KEGG for signal transduction pathway enrichment. Results: Totally,1082 target genes were obtained. GO analysis demonstrated that these targets were involved in 245 biological processes,primarily including cellular process,biological regulation,regulation of biological process and otherwise. The molecular function mainly comprised protein heterodimerization activity and I-SMAD binding,and enrichment analysis by KEGG database indicated that these target genes were functionally involved in signal pathways in MAPK,T cell receptor,Wnt and chemokine as well as inflammation. Conclusion: We successfully predicted the target genes of miR-201-3p,some of which may be involved in airway inflammation and remodeling during asthma attack.
出处 《皖南医学院学报》 CAS 2015年第6期511-517,共7页 Journal of Wannan Medical College
基金 国家自然科学基金项目(81172790) 皖南医学院中青年科研基金项目(WK201514) 国家级大学生创新创业训练项目(201310368022 201310368031) 省级大学生创新创业训练项目(AH201310368090 AH201410368089) 校级大学生科研基金项目(WK2013S16)
关键词 miR-201-3p 靶基因 生物信息学 哮喘 炎症 气道重塑 miR-201-3p target genes bioinformatics asthma inflammation airway remodeling
  • 相关文献

参考文献20

  • 1Reddel HK, Hurd SS, Fitzgerald JM. World Asthma Day. GINA 2014:a global asthma strategy for a global problem[J]. Int J Tu- berc Lung Dis,2014,18(5) :505 -506.
  • 2Robinson D, Hamid Q, Bentley A, et al. Activation of CD4 + T cells,increased TI-I2-type cytokine mRNA expression, and eosino- phil recruitment in bmnchoalveolar lavage after allergen inhalation challenge in patients with atopic asthma [ J ]. J Allergy Clin Immu- no1,1993,92(2) :313 -324.
  • 3Wills-Karp M. Immunologic basis of antigen-induced airway hyper- responsiveness[ J]. Annu Rev Immunol, 1999,17:255 - 281.
  • 4Seguin RM, Ferrari N. Emerging oligonucleotide therapies for asth- ma and chronic obstructive pulmonary disease [ J ]. Expert Opin In- vestig Drugs ,2009,18 (10) : 1505 - 1517.
  • 5Li DF, Tian J, Guo X, et al. Induction of microRNA-24 by HIF-1 protects against ischemic injury in rat cardiomyocytes [ J ]. Physiol Res ,2012,61 (6) :555 - 565.
  • 6姜玉新,马玉成,李朝品.尘螨Ⅱ类改组变应原对哮喘小鼠免疫治疗的效果[J].山东大学学报(医学版),2012,50(10):50-55. 被引量:7
  • 7Moschos SA, Williams AE, Perry MM, et al. Expression profiling in vivo demonstrates rapid changes in lung microRNA levels following lipopolysaccharide-induced inflammation but not in the anti-in- flammatory action of glucocorticoids[ J]. BMC Genomics,2007,8 : 240.
  • 8Rodfiguez A, Vigorito E, Clare S, et al. Requirement of bic/microR- NA-155 for normal immune function [ J ]. Science, 2007, 316 (5824) :608 -611.
  • 9张莺莺,钟民,张梦莹,吕坤.过敏性哮喘患者外周血CD4^+ T细胞中miR-155的表达及临床意义[J].细胞与分子免疫学杂志,2012,28(5):540-543. 被引量:12
  • 10Tomankova T, Petrek M, Kriegova E. Involvement of microRNAs in physiological and pathological processes in the lung [ J ]. Respir Res ,2010,11 : 159.

二级参考文献26

  • 1Guoping Li,Zhigang Liu,Nanshan Zhong,Bin Liao,Ying Xiong.Therapeutic Effects of DNA Vaccine on Allergen-Induced Allergic Airway Inflammation in Mouse Model[J].Cellular & Molecular Immunology,2006,3(5):379-384. 被引量:9
  • 2Larche M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma [ J]. J Allergy Clin Immunol, 2003, 111 (3) :450 -463.
  • 3Sonkoly E, Wei T, Janson PC, et al. MicroRNAs: novel regulators involved in the pathogenesis of Psoriasis? [ J/OL]. PLoS One, 2007, 2(7) : e610.
  • 4Du C, Liu C, Kang J, et al. MicroRNA miR-326 regulates TH-17 dif- ferentiation and is associated with the pathogenesis of multiple sclerosis [J]. Nat Immunol, 2009, 10(12) : 1252 -1259.
  • 5Cobb BS, Nesterova TB, Thompson E, et al. T cell lineage choice and differentiation in the absence of the RNase III enzyme Dicer[ J]. J Exp Med, 2005, 201 (9) : 1367 - 1373.
  • 6Muljo SA, Ansel KM, Kanellopoulou C, et al. Aberrant T cell differ-entiation in the absence of Dicer [ J ]. J Exp Med, 2005, 202 (2) : 261 - 269.
  • 7Banerjee A, Schambach F, DeJong CS, et al. Micro-RNA-155 inhib- its IFN-γ signaling in CIM+ T cells[J]. Eur J Immunol, 2010, 40 (1) : 225 -231.
  • 8Kohlhaas S, Garden OA, Scudamore C, et al. Cutting edge: the Foxp3 target miR-155 contributes to the development of regulatory T cells[ J]. J Immunol, 2009, 182 (5) : 2578 - 2582.
  • 9Lu LF, Thai TH, Calado DP, et al. Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein[J]. Immunity, 2009, 30(1): 80-91.
  • 10Yan C,J Bio Chem,1999年,274卷,143页

共引文献23

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部