大黄鱼serC基因的克隆、鉴定以及信息学分析

Identification and Characterization of A Phosphoserine Aminotransferase 1 cDNA in Pseudosciaena crocea

磷酸丝氨酸转氨酶1(serC)是生物L-丝氨酸合成途径中的关键酶,能够直接催化3-磷酸丝氨酸的合成,以影响多种前体物质的合成代谢,目前该基因在海洋鱼类中的研究未见报道。本研究通过对大黄鱼全基因组数据库的比对与分析,鉴定并克隆了大黄鱼serC基因,测序验证其全长。该基因c DNA全长1 581 bp,其中开放阅读框1 263 bp,共编码420个氨基酸。将大黄鱼的serC基因氨基酸序列与其它物种进行多重序列比对,结果发现大黄鱼的serC氨基酸序列比较保守。系统发育树表明大黄鱼serC基因与三刺鱼的亲缘关系最为接近。大黄鱼serC基因的组织差异表达分析采用半定量PCR技术,结果表明serC基因在大黄鱼肝脏组织中表达量最高,在脑与鳍组织中表达较高,其余组织中表达量都比较低。本研究分析了大黄鱼serC基因的表达特征与进化地位,为serC基因家族的进一步研究提供了基础资料。

Phosphoserine aminotransferase 1 is a key enzyme in synthesing L-serine. This enzyme has great influence on metabolism of many precussor substances by catalyze 3-phosphoserine, which has not been reported in marine teleost. In this study, a phosphoserine aminotransferase 1 （serC） gene from large yellow croaker （Pseudosciaena crocea） was isolated and characterized by searching and aligning the whole genome database of P.crocea. The overall cDNA length is 1 581 bp, containing an open reading frame of 1 263 bp, encoding 420 amino acids. According to the multiple alignment analysis, the amino acids sequence of serC was relatively conserved＇ Phylogenetic tree is built to show the genetic distance of serC with other species. The result showed serC from P. crocea is most genetically closed to Gasterosteus aculeatus with a 91.46% similarity. RT-PCR was conducted to check serC in tissue expression. The result showed the most abundant serC expression is in liver, and brain and fin tissue have high expression. In this study serC gene of P. crocea was identified and characterized, which might contribute to the further research on the serC family genes.