晶状体胚胎发育过程中促红细胞生成素的作用

Significance of erythropoietin for the embryonic development of the lens

背景:有研究发现促红细胞生成素对视网膜胚胎发育具有保护作用,但对晶状体胚胎发育的影响的研究尚少有报道。目的:通过观察不同胚胎发育期Wistar大鼠晶状体组织中促红细胞生成素的表达差异,探讨促红细胞生成素在晶状体胚胎发育过程中的作用和意义。方法:选取清洁级Wistar母鼠孕10 d、孕12 d、孕14 d、孕16 d、孕18 d、孕20 d各5只,每只孕鼠氯胺酮麻醉后剖腹随机取胎鼠2只,分离各组的胚鼠眼球组织,并制作石蜡切片,用免疫组织化学法、实时定量PCR方法检测大鼠晶状体组织中促红细胞生成素蛋白及其促红细胞生成素信使核糖核酸的表达。结果与结论:不同胚胎期晶状体组织中均有促红细胞生成素阳性表达,孕10 d至孕16 d,表达量逐渐升高,孕16 d至孕20 d,表达量逐渐降低,孕16 d时达到峰值,各胚胎期促红细胞生成素表达量采用单因素方差分析,差异有显著性意义。结果说明Wistar大鼠胚胎发育过程中,晶状体中促红细胞生成素呈现由低到高、再由高到低的表达量的变化趋势,其表达规律提示促红细胞生成素可能参与了晶状体的胚胎发育过程并在其中发挥保护和调节作用。

BACKGROUND：Studies have found that erythropoietin has a protective effect on embryonic development of the retina, but there are rare studies concerning the embryonic development of the lens. OBJECTIVE： To observe the difference in erythropoietin expression in the lens from Wistar rats at different embryonic periods and to investigate the effect and significance of erythropoietin in the embryonic development of the lens. METHODS：Clean Wistar rats with pregnancy for 10 days （n=5）, 12 days （n=5）, 14 days （n=5）, 16 days （n=5）, 18 days （n=5）, 20 days （n=5） were randomly colected and divided into six groups. Every two embryonic ratsof the different 30 pregnant rats were obtained randomly by the caesarean operation under ketamine-induced anesthesia. The eye tissues of al the embryonic rats were isolated and cut into sections. The expression of erythropoietin protein and mRNA in rat lens was detected by immunohistochemistry and reverse t ranscription-PCR, respectively. RESULTS AND CONCLUSION：Erythropoietin was distinctly expressed at the six different embryo stages, and the expression of erythropoietin protein and mRNA gradualy increased from embryonic day 10 to embryonic day＆amp;nbsp;16, and decreased from embryonic day 10 to embryonic day 20. There were significant differences between the six groups. These findings indicate that the expression of embryonic appears in a low to high to low fashion during the embryonic development of Wistar rats, which may be closely associated with the developing procedure of lens.