镍钛合金丝对大鼠心肌细胞肌浆网钙泵及雷尼丁受体的影响

Effect of nickel-titanium wire on the expression of sarcoplasmic reticulum Ca^(2+)-ATPase and ryanodine receptor in rat myocardial cells

背景:虽然镍钛合金封堵器置入治疗先天性心脏病具有较好的临床效果,但后期发生心律失常并发症的概率较大。目的:观察镍钛合金对大鼠心肌细胞肌浆网钙泵及雷尼丁受体表达的影响。方法:取30只SD大鼠,随机均分为2组,实验组于心尖部植入镍钛合金丝,对照组不做任何处理特殊处理,术后1,3,6个月取材,采用RT-PCR检测心肌细胞肌浆网钙泵与雷尼丁受体基因的表达,采用免疫组化检测心肌细胞肌浆网钙泵及雷尼丁受体蛋白的表达,采用苏木精-伊红染色检测炎症反应情况。结果与结论:实验组镍钛合金植入大鼠心肌后,炎症细胞浸润,引发炎症反应,伴纤维组织增生,随着植入时间延长,炎症反应逐渐消失;对照组未见炎细胞浸润。两组术后不同时间点的心肌细胞肌浆网钙泵、雷尼丁受体基因表达比较差异无显著性意义,相应的蛋白表达比较差异也无显著性意义。表明镍钛合金丝不影响心肌细胞肌浆网钙泵、雷尼丁受体的表达,提示镍钛合金封堵器相关心律失常可能与肌浆网钙泵、雷尼丁受体蛋白表达异常机制关系不大。

BACKGROUND： Although the nickel-titanium occluder in the treatment of congenital heart disease has a better clinical effect,arrhythmia will be more likely to develop in late stage. OBJECTIVE： To investigate the effect of nickel-titanium wire on expression of sarcoplasmic reticulum Ca^2＋-ATPase and ryanodine receptor of rat myocardial cells. METHODS： Thirty Sprague-Dawley rats were obtained and randomly divided into two groups： experimental and control groups. The nickel-titanium wire was implanted to the apex of heart of rats in the experimental group. Rats in the control group received no special treatment. Rat mycardial cells were harvested at the 1^th,3^rd and 6^th months after operation. The gene and protein expressions of sarcoplasmic reticulum Ca^2＋-ATPase and ryanodine receptor of rat myocardial cells were detected using RT-PCR and immunohistochemistry,respectively. Theinflammatory reactions were detected using hematoxylin-eosin staining. RESULTS AND CONCLUSION： After the nickel-titanium wire was implanted into the rat myocardium,inflammatory reaction was induced by inflammatory cell infiltration in the experimental group,with hyperplasia of fibrous tissue. The inflammatory reaction gradually disappeared as the implanted time extended. No inflammatory cell infiltration was visible in the control group. There was no significant difference in the gene and protein expressions of sarcoplasmic reticulum Ca^2＋-ATPase and ryanodine receptor of rat myocardial cells at different time points after operation between these two groups. It showed that nickel-titanium wire had no influence on the expressions of sarcoplasmic reticulum Ca^2＋-ATPase and ryanodine receptor of rat myocardial cells. These results suggest that nickel-titanium occluder-related arrhythmia may have little relationship with abnormal protein expression of sarcoplasmic reticulum Ca^2＋-ATPase and ryanodine receptor.