三种温控脱细胞载体的制备与性能评估

Preparation and performance of three kinds of thermoresponsive acellular carriers

背景:设计、制备新型温敏高分子三维细胞支架是目前高分子科学研究的热点之一。目的:制备3种不同的温控脱细胞载体,并评估其性能。方法:分别制备共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架,检测3组支架的比表面积、温敏性能、孔隙率、孔径及细胞相容性。结果与结论:共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的比表面积分别为135,386,421 m2/g,最低临界溶解温度分别为30,28.5,29.5℃,细胞毒性反应分别为2级、2级、1级,说明3种支架均具备温度敏感特性,而且最低临界溶解温度相近;大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的细胞相容性明显优于另两种支架。大孔共聚异丙基丙烯酰胺温控脱细胞支架、大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架的孔隙率与孔径大于共聚异丙基丙烯酰胺温控脱细胞支架(P<0.05),说明大孔共聚异丙基丙烯酰胺温控脱细胞支架与大孔共聚异丙基丙烯酰胺交联醛基化海藻酸钠温控脱细胞支架具有更加明显的孔洞结构。

BACKGROUND： To design and fabricate a novel three-dimensional thermoresponsive polymer cell scaffold is one of the hot topics in the research of polymer science. OBJECTIVE： To prepare three different kinds of thermoresponsive acellular carriers and to evaluate their performance. METHODS： The copolymer N-isopropylacrylamide temperature acellular scaffold,macroporous copolymer N-isopropylacrylamide temperature acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold were prepared. The specific surface area,thermoresponsive performance,porosity,pore size and biocompatibility of these three groups of scaffolds were determined. RESULTS AND CONCLUSION： The specific surface area of copolymer N-isopropylacrylamidethermoresponsive acellular scaffold,macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive cellular scaffold was respectively 135,386,421 m^2/g. The lower critical solution temperature was 30,28.5,29.5 ℃. The cell toxicity reaction was respectively grade 2,2,1. These indicators showed that the three kinds of scaffolds were provided with a temperature-sensitive characteristics and similar lower critical solution temperature. The biocompatibility of macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold was significantly better than the other two scaffolds. The porosity and pore size of macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold were greater than those of the copolymer N-isopropylacrylamide thermoresponsive acellular scaffold（P〈0.05）. These results demonstrate that macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold have more obvious pore structure.