电针不同穴位对模拟失重效应大鼠HPA轴相关激素及神经递质的影响

Effect of electroacupuncture at different acupoints on hormones and neurotransmitters of hypothalamic-pituitary-adrenal axis in rats under simulated weightlessness

目的:探寻电针不同穴位对失重条件下机体内分泌轴及相关神经递质的影响及变化规律。方法:40只雄性清洁级Wistar大鼠随机分为正常组、尾吊组、电针内关组、电针三阴交组,每组10只。尾吊组、电针内关组、电针三阴交组大鼠尾部悬吊模拟失重效应。正常组常规饲养;尾吊组尾吊28天;电针内关组大鼠尾吊的同时,予以电针双侧"内关"30min,隔日1次,共治疗14次;电针三阴交组大鼠尾吊的同时,予以电针双侧"三阴交"穴30min,隔日1次,共治疗14次。均于4周后取材。采用放射免疫法检测各组大鼠下丘脑促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH)、垂体促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、血清中皮质酮(corticosterone,CORT)及下丘脑中5-羟色胺(5-hydroxy tryptamine,5-HT)、多巴胺(dopamine,DA)、去甲肾上腺素(norepinephrine,NE)的含量。结果:与正常组比较,尾吊组大鼠垂体组织ACTH含量降低(P<0.05),下丘脑中5-HT含量明显降低(P<0.01);电针"内关"组大鼠下丘脑组织中CRH、5-HT的含量降低(P<0.01,P<0.05);电针三阴交组大鼠下丘脑组织中CRH、5-HT的含量降低(P<0.01,P<0.05),血清中CORT的含量降低(P<0.05)。与尾吊组比较,电针内关组垂体组织ACTH含量降低(P<0.05);电针三阴交组大鼠下丘脑组织中CRH含量、垂体组织ACTH含量、血清中CORT含量亦降低(P<0.05,P<0.01)。与电针内关组比较,电针三阴交组大鼠血清CORT的含量明显降低(P<0.05)。结论:电针可以调节尾吊大鼠下丘脑中5-HT的含量,抑制尾吊大鼠HPA轴的兴奋状态,电针"三阴交"较电针"内关"抑制作用更明显,但对NE、DA的作用并不明显。

Objective To explore the change pattern of hypothalamic-pituitary-adrenal （HPA） axis and related neurotransmitters under simulated weightlessness. Methods A total of 40 clean-grade male Wtstar rats were randomly divided into a normal group, a tail-suspension group, an electroacupuncture （EA） at Neiguan （PC 6） group, an EA at Sanyinjiao （SP 6） group, 10 rats in each group. Rats in the tail-suspension group, EA at ＂Neiguan＂ （PC 6） group and EA at ＂Sanyinjiao＂ （SP 6） group were treated with tail suspension to simulate weightlessness effect. Rats in the normal group were treated with normal diet, Rats in the tail-suspension group were treated with tail suspension for 28 d. During the time of tail suspension, rats in the EA at ＂Neiguan＂ （PC 6） group were treated with EA at ＂Neiguan＂ （PC 6）, 30 rain per treatment, once every two days for 14 treatments,while rats in the EA at ＂Sanyinjiao＂ （SP 6） group were treated with EA at ＂Sanyinjiao＂ （SP 6）, 30 min per treatment, once every two days for 14 treatments. Samples were all collected after 4 weeks. The contents of cortico- tropin releasing hormone （CRH）, adrenocorticotropic hormone （ACTH）, cortieosterone （CORT） in as well as 5- hydroxy tryptamine （5-HT）, dopamine （DA）, norepinephrine （NE） Were measured by using radioimmunoassay. Results Compared with the normal group, in the tail-suspension group the content of ACTH in pituitary was sig- nificantly decreased （P〈0.05）, and the content of 5 HT in hypothalamus was significantly decreased （P〈0.01） ; the content of CRH and 5-HT in hypothaiamus was significantly decreased （P〈0.01, P〈0.05） in the EA at ＂Neiguan＂ （PC 6） group; the content of CRH and 5-HT in hypothalamus was significantly decreased （P〈0.01）, and the content of CORT in serum was significantly decreased （P〈0.05） in the EA at ＂Sanyinjiao＂ （SP 6） group. Compared with the tail-suspension group, the content of ACTH in hypothatamus was significantly decreased （P〈 0.05） in the EA at ＂Neiguan＂ （PC 6） group; the content of CRH, ACTH and CORT was significantly decreased （P〈0.01, P〈0. 05） in the EA at ＂Sanyinjiao＂ （SP 6） group. Compared with the EA at ＂Neiguan＂ （PC 6） group, the content of CORT was decreased （P〈0. 05） in the EA at ＂Sanyinjiao＂ （SP 6） group. Conclusion EA can regulate the content of 5-HT in hypothalamus in tail-suspension rats, inhibit the hyperactivity of the HPA axis, in which EA at＂ Sanyinjiao＂ （SP 6） had more significant effects than ＂Neiguan＂ （PC 6）, but no obvious effects on NE and DA were observed.