LH受体基因rs13405728位点多态性与卵巢慢反应的相关性

Association of rs13405728 polymorphism of LHR gene with slow ovarian response

目的探讨黄体生成素受体（1uteinizing hormone receptor，LHR）基因多态性与接受辅助生殖技术治疗妇女卵巢慢反应的关系。方法提取236例接受体外受精／单精子胞浆注射-胚胎移植的不孕患者外周血白细胞基因组DNA，通过基因测序进行LHR基因rs13405728位点基因分型。结果LHR基因的rs13405728位点存在AA（52．54％）、AG（40．68％）和GG（6．78％）3种基因型。窦卵泡数（antral folliclecount，AFC）、抗苗勒管激素（anti—Mtillerian hormone，AMH）在不同基因型患者间的差异无统计学意义（P〉0．05）。GG基因型患者中慢反应的发生率低于GA和AA基因型患者（P〈0．05）。3种基因型患者的卵泡刺激素用量、扳机日卵泡直径≥14mm的卵泡数、获卵数、成熟卵数、可利用胚胎率和临床妊娠率的差异无统计学意义（P〉0．05）。卵巢慢反应的发生与rs13405728位点的基因型、促性腺激素（gonadotropin，Gn）启动剂量及黄体生成素使用量呈独立相关（P〈0．05）。结论rs13405728位点基因多态性与卵巢慢反应相关；卵巢慢反应和低反应的发生机制可能不同。

Objective To assess the association of rs13405728 polymorphism of luteinizing hormone receptor （LHR） gene with slow ovarian response during assisted reproductive technology（ART）. Methods Two hundred and thirty-six women were enrolled and grouped according to their genotypes. The rs13405728 polymorphism was genotyped by DNA sequencing. Results No signifiicant difference was found in antral follicle count and anti-Mullerian hormone between the three genotypes（P〉 0.05）. The incidence of slow response in genotype GG was lower than in the other two genotypes（P〈0. 05）. There was no significant difference in the amount of follicle stimulating hormone required, the number of follicles ≥14 mm on human chorionic gonadotrophin day, oocytes, mature oocytes, available embryos, and the clinical pregnancy rate among the three genotypes（P〉0.05）. There was an independent correlation between slow ovarian response with the genotypes of rs13405728, the initial dose of gonadotropin, and the dose of luteinizing hormone required（P〈 0.05）. Conclusion Rs13405728 of the LHR gene may be associated with slow ovarian response in ART. Various mechanisms may be involved in the poor response and slow response.