摘要
目的研究福建汉族人群D1S1656的遗传多态性及其在亲权鉴定中的应用价值。方法应用荧光标记复合扩增系统对日常检案中收集的521名福建汉族无关个体血样DNA进行PCR扩增,用ABI 3130基因分析仪对扩增产物进行毛细管电泳,用Gene Mapper v3.1软件进行基因分型,统计分析D1S1656基因座的多态信息,并与其他5个群体进行比较。结果在D1S1656基因座发现17个等位基因,在福建汉族人群中的个体识别能力为0.948,非父排除率为0.665。结论 D1S1656基因座在福建汉族人群具有高度多态性,在亲权鉴定中具有重要应用价值。
Objective To investigate the genetic polymorphism of D1S1656 STR loci in Han population from Fujian province and to study its application in paternity testing. Methods Fluorescent dye labeling multiplex STR-PCR,capillary electrophoresis and Gene Mapper v3.1 software were adopted in genotyping 521 unrelated samples. D1S6156 STR database was established and analyzed.Population comparison was conducted between Han population in Fujian province and 5 other population. Results We observed the distribution of D1S1656 STR loci meet Hardy-Weinberge equilibrium and was not different from other 5 population（χ^2test,P〉0.05）. The power of discrimination was 0.948, and the power of exclusion was 0.665. Conclusion The D1S1656 loci was relatively highly genetic polymorphic in Fujian Han population and could be used for paternity testing.
出处
《中国卫生产业》
2015年第9期3-4,共2页
China Health Industry
基金
福建省临床重点专科建设项目资助[闽卫科教(2012)149号]
