食品中有害微生物的常规检验方法分析

Analysis of Conventional Test Methods of Harmful Microorganisms in Food

食品安全直接关系着人民群众的身体健康和生命安全。近年来,受经济利益的驱使,食品安全事故屡见报端,食源性疾病的发生率不断升高,食品微生物指标不合格等现象也引起了人们的广泛关注,成为亟待解决的重点问题之一。聚合酶链式反应(PCR)技术是基于分子生物学技术的用于检验食品中有害微生物的常规方法之一,其特异性和敏感性高,同时兼具检测速度快、准确性高等优点,在食品有害微生物检测中发挥了重要作用。本文介绍了PCR技术检测食品有害微生物的主要流程与现状,就目前该技术应用于食品有害微生物检测中存在的问题及其解决措施进行了探讨,旨在为食品有害微生物检验提供借鉴和参考。

The food safety is directly related to people＇s health and life safety. In recent years, driven by economic interests, the food safety accident frequently appear in newspapers, increased incidences of food borne diseases, food microbiology indicators unqualified phenomenon has caused people＇s extensive concern, become one of the key problems to be solved. Polymerase chain reaction（PCR） technology is a molecular biology technique for one of the conventional method of harmful microorganisms in food based on the test, the high specificity and sensitivity, fast detection speed, simultaneously has the advantages of high accuracy, the food is harmful to play an important role in microbial detection. This paper introduces PCR technology to detect food harmful microorganism mainly process and current situation, at present the technology applied in food is harmful to the presence of microbial detection problems and solving measures were discussed, aimed to harmful microorganism inspection and provide the reference for food.