依达拉奉对大鼠脑缺血再灌注损伤的保护作用研究

Study on the Protective Effect of Edaravone on Cerebral Ischemia Reperfu-sion Injury in Rats

目的探讨依达拉奉对大鼠脑缺血再灌注损伤的保护作用。方法将36只SD大鼠随机分为假手术组、NS组及依达拉奉组,制备SD脑缺血再灌注模型,依达拉奉组在造模后即刻和12h分别给予依达拉奉腹腔注射,假手术组和NS组分别给予生理盐水腹腔注射,比较3组神经功能缺损评分及SOD、MDA含量。结果与假手术组比较,NS组与依达拉奉组神经功能缺损评分分别为(2.74±0.56)、(1.38±0.22)分均明显升高(P<0.05),而与NS组比较,依达拉奉组神经功能缺损评分则明显下降(P<0.05);与假手术组比较,NS组与依达拉奉组SOD活性分别为(113.29±7.61)、(144.93±10.48)U/m L明显下降(P<0.05),而与NS组相比较,依达拉奉组SOD活性明显增高(P<0.05);与假手术组比较,NS组与依达拉奉组MDA水平分别为(3.71±1.24)、(2.25±0.98)nmol/m L明显升高(P<0.05),而与NS组相比较,依达拉奉组MDA水平明显下降。结论依达拉奉对大鼠脑缺血再灌注损伤具有明显的保护作用。

Objective To investigate the protective effect of edaravone on cerebral ischemia reperfusion injury in rats. Methods 36 SD rats were randomly divided into sham operation group, NS group and edaravone group, preparation of SD in cerebral ischemia reperfusion model group, edaravone immediately after modeling and 12 h were given intraperitoneal injection of edaravone, sham operation group and NS group were given intraperitoneal injection of normal saline, neural function defect score and SOD, the content of MDA of 3 groups. Results The Compared with the sham operation group, NS group and edaravone group, the score of neural function defect were（2.74±0.56）,（1.38±0.22） were significantly increased（P〈0.05）, compared with NS group, the score of neural function defect edaravone group decreased significantly（P〈0.05）; compared with the sham operation group NS group and edaravone group, the activity of SOD were（113.29±7.61）,（144.93±10.48） U/ml decreased significantly（P〈0.05）, and compared with the NS group, edaravone group SOD activity obviously increased（P〈0.05）; compared with the sham operation group, NS group and edaravone group MDA horizontal don＇t as（3.71 ±1.24）,（2.25 ±0.98） nmol/ml increased significantly（P〈0.05）, and compared with the NS group, the levels of MDA of edaravone group decreased significantly. Conclusion edaravone on cerebral ischemia reperfusion injury has obvious protective effect.