pH调控的含糖聚合物与蛋白质的相互作用

pH-Dependent Specific Protein Recognition Ability of Glycopolymers

采用连续投料法合成了两嵌段含糖聚合物——聚甲基丙烯酸葡萄糖氨基乙基酯-b-聚甲基丙烯酸-2-二甲氨基乙酯-co-甲基丙烯酸-2-二乙氨基乙酯(PGAMA-b-PDMAEMA-co-DEAEMA,PGME),并用核磁共振谱(1H NMR)和凝胶渗透色谱(GPC)确认了含糖聚合物PGME的化学组成及分子量.采用浊度法研究了含糖聚合物PGME与蛋白质的相互作用情况,通过改变环境p H值调控PGME对蛋白质的相互作用:当p H=8.3时,PGME只能与刀豆球蛋白A(Con A)结合,表现出PGME与Con A特异性结合的行为;而当p H=7.3时,PGME表现出与蛋白质的非特异性结合,说明通过改变环境p H值可调控PGME对蛋白质的相互作用.用动态光散射和原子力显微镜(AFM)表征了PGME水溶液聚集体.

A new di-block glycopolymer, poly （gluconamidoethylmethacrylate） -b-poly [ 2- （diethylamino） ethyl methacrylate ] -co-poly [ 2- （ dimethylamino ） ethyl methacrylate ] [ PGAMA-b-P （ DMAEMA-co-DEAEMA ）, PGME] was synthesized. The chemical composition and the molecular weight of the glycopolymer were characterized by nuclear magnetic resonance （^1H NMR） and gel permeation chromatography （GPC）. The interactions of PGME and proteins were investigated by monitoring the turbidity value of the mixture solutions. PGME showed the specific biomolecular recognition ability with Concanavalin A compared with bovine serum albumin （BSA） at pH = 8.3. When the environmental pH value changed to 7.3, the ability of PGME specific biomolecular recognition was suppressed and PGME exhibited the behavior of non-specific binding with bovine serum albumin and Concanavalin A. Dynamic light scattering and atomic force microscopy （AFM） tests were performed to investigate the properties of the PGME-protein complex aggregations, it would be a promising to design pH-sensitive drug delivery system of saccharides.