ALCAM/CD166在乳腺癌组织中表达及意义以及其与Bcl-2、Ki-67的关系

Expression of ALCAM/CD166 in Breast Infiltrative Ductal Cancer Tissues and Its Relation with Bd-2 or Ki-67

目的研究乳腺浸润性导管癌组织中活化白细胞黏附分子(ALCAM/CD166)的表达及其与凋亡抑制蛋白Bcl-2及增殖细胞核抗原Ki-67的关系,探讨ALCAM/CD166在乳腺浸润性导管癌发生、发展中的作用。方法应用免疫组织化学Elivision^(TM) Plus二步法检测96例乳腺浸润性导管癌组织和30例癌旁非瘤乳腺上皮组织中ALCAM/CD166及Bcl-2、Ki-67蛋白表达的情况,分析ALCAM/CD166蛋白表达与乳腺浸润性导管癌患者的年龄、肿瘤直径、组织病理学分级、淋巴结转移及TNM分期的关系以及其与Bcl-2、Ki-67的相关性。结果 1在96例乳腺浸润性导管癌中ALCAM/CD166蛋白表达阳性率为79.2%(76/96),明显高于其在癌旁非瘤乳腺上皮组织中的10.0%(3/30),差异有统计学意义(P<0.01)。2 ALCAM/CD166蛋白阳性表达与乳腺浸润性导管癌患者的年龄、肿瘤直径、组织病理学分级及TNM分期均无关(P>0.05),与腋窝淋巴结转移有关(P<0.05)。3乳腺浸润性导管癌组织中ALCAM/CD166蛋白表达与Bcl-2蛋白表达呈正相关(rs=0.307,P=0.001),而与Ki-67蛋白表达无关(rs=0.064,P=0.475)。结论 ALCAM/CD166蛋白表达与乳腺浸润性导管癌细胞凋亡及转移有关,可能成为判断肿瘤生物学行为和患者预后的重要参考指标之一。

Objective To investigate expressions ofALCAM/CD166, Bcl-2, and Ki-67 in breast infiltrative ductal cancer tissues, so as to assess the role of ALCAM/CD166 protein in the carcinogenesis and progression of breast infiltrative ductal cancer. Methods The expressions ofALCAM/CD166, Bcl-2, and Ki-67 proteins were examined by immunohistochemistry （ElivisionTM Plus） in 96 breast infiltrative ductal cancer specimens and 30 adjacent normal tissues of breast cancer specimens （control group）. The relation between ALCAM/CD166 protein expression and patient＇s age, tumor diameter, histopathologic grade, axillary lymph node metastasis, or TNM stage of breast infiltrative ductal cancer was analyzed, and the correlation between ALCAM/CD166 expression and Bcl-2 or Ki-67 was analyzed too. Results （1） In 96 cases of breast infiltrative ductal cancer, the positive rate of ALCAM/CD 166 protein expression was 79.2% （76/96）, which was significantly higher than that in the control group [ 10.0% （3/30）, P〈0.011. （2） In the breast infiltrative ductal cancer tissues, the expression of ALCAM/CD166 was related to axillary lymph node metastasis （P〈0.05）, but was not related to patient＇s age, tumor diameter, histopathologic grade, and TNM stage （P〉0.05）. （3） The ALCAM/CD166 protein expression was positively related to Bcl-2 （rs=0.307, P=0.001） and not related to Ki-67 （rs=0.064, P=0.475）. Conclusion ALCAM/CD 166 protein expression might be related to the apoptosis and metastasis of breast infiltrative ductal cancer cells and it could serve as an important marker for predicting biological behavior and prognosis of tumor.