槲皮素对人肺腺癌NCI-H1395细胞凋亡的影响

Effects of Quercetin on Human Lung Cancer NCI-H1395 Cell Apoptosis

目的：研究槲皮素对人肺腺癌NCI-H1395细胞凋亡的影响。方法：采用CCK-8法检测0-200μmol/L槲皮素作用12、24、48 h对NCI-H1395细胞增殖的影响;应用Hochest33258染色法和流式细胞仪检测0、20、50、100μmol/L槲皮素作用24 h对NCI-H1395细胞凋亡的影响;检测100μmol/L槲皮素对经半胱氨酸天冬氨酸蛋白酶8（Caspase-8）、Caspase-9、Caspase-3抑制剂处理后NCI-H1395细胞凋亡的影响。结果：槲皮素可抑制NCI-H1395细胞的增殖,且与浓度和时间呈正相关。20、50、100μmol/L槲皮素可诱导NCI-H1395细胞凋亡,凋亡率分别为（18.6±4.1）%、（39.1±4.5）%、（58.2±3.5）%。抑制Caspase-8、Caspase-3激活可明显降低槲皮素对细胞的抑制作用（P〈0.05）。结论：槲皮素可抑制NCI-H1395细胞增殖,诱导其凋亡,其作用可能与凋亡外源通路Caspase-8和Caspase-3的激活有关。

OBJECTIVE：To investigate the effects of quercetin on human lung cancer NCI-H1395 cell apoptosis. METHODS：CCK-8 was used to detect the effects of 0-200 μmol/L quercetin on human lung cancer NCI-H1395 cell proliferation after treated for 12,24 and 48 h. Hochest33258 staining and flow cytometry were used to detect the effects of 0,20,50,100 μmol/L quercetin on NCI-H1395 cell apoptosis after treated for 24 h. The effects of 100 μmol/L quercetin on NCI-H1395 cell apoptosis was investigated after treated with Caspase-8,Caspase-9,Caspase-3 inhibitor. RESULTS：Quercetin could inhibit NCI-H1395 cell proliferation in dose and time-dependent manner. 20,50,100 μmol/L quercetin could induce the apoptosis of NCI-H1395 cell,and apoptotic rates were（18.6 ± 4.1）%,（39.1 ± 4.5）% and（58.2 ± 3.5）%. Caspase-8 and Caspase-3 activation inhibition could obviously weaken the inhibitory effects of quercetin on cell（P〈0.05）. CONCLUSIONS：Quercetin can inhibit NCI-H1395 cell proliferation and induce cell apoptosis,which is related to the external way of cell apoptosis through activating Caspase-8 and Caspase-3.